小鼠毛囊发育中细胞增殖区及干细胞巢的形成演化

doi:10.3760/cma.j.issn.0412?4030.2018.03.018

中华皮肤科杂志 ›› 2018, Vol. 51 ›› Issue (3): 230-233.doi: 10.3760/cma.j.issn.0412?4030.2018.03.018

小鼠毛囊发育中细胞增殖区及干细胞巢的形成演化

王洪阳,王海涛,李树伟

塔里木大学生命科学学院

收稿日期:2017-04-13 修回日期:2017-12-09 出版日期:2018-03-15 发布日期:2018-03-06
通讯作者: 李树伟 E-mail:xj_lsw@126.com
基金资助:
国家自然科学基金;小鼠触须毛囊外根鞘的体外再生培养及形态学观察

Formation and evolution of hair follicle stem cell proliferative region and stem cell niche during the development of murine hair follicles

Hong-Yang ¹,WANG HaiTao¹,Shu-Wei LI

Received:2017-04-13 Revised:2017-12-09 Online:2018-03-15 Published:2018-03-06
Contact: Shu-Wei LI E-mail:xj_lsw@126.com
Supported by:
National Natural Science Foundation of China

摘要/Abstract

摘要： 目的根据不同发育时期小鼠触须毛囊形态结构以及毛囊干细胞增殖区定位，初步分析毛囊隆突部干细胞巢的形成与意义。方法采用干细胞增殖标记物5-溴-2-脱氧尿嘧啶（EdU）体内局部注射法，标记不同生长发育阶段的SPF级健康昆明远交系小鼠触须毛囊，24 h后获取完整触须毛囊，OCT包埋，冰冻切片，HE染色。对EdU标记的切片行免疫荧光染色，检测毛囊细胞增殖活跃区域，并采用抗干细胞转录因子Sox2抗体对毛囊进行免疫荧光染色。结果毛囊在发育过程中形态发生变化，成熟毛囊可以明显看出毛囊隆突部位。自出生后第1天至第15天，小鼠毛囊细胞增殖活跃区从最初的真皮乳头渐渐转移至毛囊球部内外根鞘部位，同时沿内外根鞘向上迁移，在毛囊中上部形成毛囊隆突，并且Sox2在隆突部细胞中大量表达。结论小鼠毛囊隆突部干细胞巢的祖细胞来源于毛囊真皮乳头，毛囊隆突形成后细胞大量表达Sox2，并进入静息状态。

关键词: 毛囊, 触须, 干细胞研究, 细胞增殖, SOXB1转录因子类, 荧光抗体技术

Abstract: Wang Hongyang, Wang Haitao, Li Shuwei College of Life Sciences, Tarim University, Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alaer 843300, Xinjiang, China Corresponding author: Li Shuwei, Email: xj_lsw@126.com 【Abstract】 Objective To preliminarily analyze the formation and significance of stem cell niche in the hair follicle bulge, based on the morphological structure of murine vibrissa follicles and localization of proliferative region of hair follicle stem cells at different developmental stages. Methods Vibrissa follicles of healthy, specific pathogen-free （SPF） outbred KM mice at different developmental stages were labelled with 5-ethynyl-2-deoxyuridine （EdU） as a marker of proliferating stem cells by in vivo local injection. After 24 hours, the complete vibrissa follicles were obtained by surgical microanatomy, and then subjected to OCT embedding, cryostat sectioning and hematoxylin-eosin （HE） staining. Sections labelled with EdU were subjected to immunofluorescence staining to determine the active proliferative region of hair follicle cells. Hair follicles were subjected to immunofluorescence staining with anti-stem cell transcription factor （Sox2） antibody. Results The morphology of hair follicles changed during the development, and mature hair follicles could be clearly observed in the hair follicle bulge. On day 1 to day 15 after birth, the active proliferative region of murine hair follicle cells was gradually transferred from the dermal papillae to the inner and outer root sheaths in the hair follicle bulb. Meanwhile, along with the inner and outer root sheaths, the actively proliferating hair follicle cells migrated upward to the middle and upper parts of hair follicles and formed the hair follicle bulge, in which the stem cell transcription factor Sox2 was abundantly expressed in cells. Conclusions Progenitor cells in the stem cell niche of the hair follicle bulge are derived from the dermal papillae of hair follicles. After the formation of the hair follicle bulge, cells start to abundantly express Sox2 and enter into the resting state.

Key words: Hair follicle, Vibrissae, Stem cell research, Cell proliferation, SOXB1 transcription factors, Fluorescent antibody technique

中图分类号:

R253

王洪阳王海涛李树伟. 小鼠毛囊发育中细胞增殖区及干细胞巢的形成演化[J]. 中华皮肤科杂志, 2018, 51(3): 230-233.

Hong-Yang WANG HaiTao Shu-Wei LI. Formation and evolution of hair follicle stem cell proliferative region and stem cell niche during the development of murine hair follicles[J]. Chinese Journal of Dermatology, 2018, 51(3): 230-233.

参考文献 9

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小鼠毛囊发育中细胞增殖区及干细胞巢的形成演化

Formation and evolution of hair follicle stem cell proliferative region and stem cell niche during the development of murine hair follicles

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