中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (10): 748-750.doi: 10.3760/cma.j.issn.0412-4030.2017.10.013

• 研究报道 • 上一篇    下一篇

成人跖疣人乳头瘤病毒分型及与临床特征的关系

董强 杨番 任英云 夏天保 尹萌萌 王泽坤 米霞 刘军连 曹晓佳 刘建军 葛芸 梁龙 吕世超   

  1. 100101 北京，安徽医科大学解放军第306 临床学院皮肤科（董强、夏天保、尹萌萌、王泽锟、米霞、刘军连、曹晓佳、刘建军、葛芸、梁龙、吕世超）；天津市武清区人民医院医疗美容科（杨番）；佛山市南海区第三人民医院皮肤科（任英云）

  • 收稿日期:2016-12-01 修回日期:2017-06-08 发布日期:2017-09-29
  • 通讯作者: 吕世超 E-mail:xtb78@163.com
  • 基金资助:
    首都临床特色应用研究（Z141107002514179）

Relationship between human papillomavirus genotypes in and clinical characteristics of adult plantar warts

Dong Qiang, Yang Fan, Ren Yingyun, Xia Tianbao, Yin Mengmeng, Wang Zekun, Mi Xia,#br# Liu Junlian, Cao Xiaojia, Liu Jianjun, Ge Yun, Liang Long, Lyu Shichao#br#   

  1. Department of Dermatology, 306th Clinical College of People′ s Liberation Army, Anhui Medical University, Beijing 100101, China（Dong Q, Xia TB, Yin MM, Wang ZK, Mi X, Liu JL, Cao XJ, Liu JJ, Ge Y, Liang L , Lyu SC）; Department of Medical Cosmetology, The People′ s Hospital of Wu Qing, Tianjin 301700, China （Yang F）; Department of Dermatology, The Third People′s Hospital, Nanhai District, Foshan City, Foshan 528200, Guangdong, China（Ren YY）
  • Received:2016-12-01 Revised:2017-06-08 Published:2017-09-29
  • Supported by:
    Capital Clinical Characteristic Application Research（Z141107002514179）

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摘要： 目的 检测成人跖疣乳头瘤病毒（HPV）型别，并探讨HPV分型与临床特征的关系。方法 采用PCR扩增、基因测序和TA克隆技术检测221例跖疣患者疣体的HPV型，记录患者的年龄、病程、症状和疣体数。结果 221例标本中，HPV阳性率为97%（215/221）。单一HPV型占96%（206/215），HPV 27、2、57和7（Alpha属）分别占44.7%（92/206）、12.1%（25/206）、7.3%（15/206）和0.5%（1/206）；HPV 65（Gamma属）占0.5%（1/206）；HPV 1、63（Mu属）占33.5%（69/206）和1.5%（3/206）。HPV 27、2和57三型感染者之间年龄、病程、疣体个数、疼痛发生率和性别比较，差异均无统计学意义。与HPV 27感染者相比，HPV 1感染者与年龄 ≤ 30岁、病程 ≤ 1年、疣体数 ≤ 2个和疼痛高发生率相关。结论 HPV 27、2、57和1是感染成人跖疣患者的主要HPV型。HPV型与患者的临床特征间存在相关性。

关键词: 乳头状瘤病毒科, 基因分型技术, 疣, PCR, 临床特征, TA克隆

Abstract: Dong Qiang, Yang Fan, Ren Yingyun, Xia Tianbao, Yin Mengmeng, Wang Zekun, Mi Xia, Liu Junlian, Cao Xiaojia, Liu Jianjun, Ge Yun, Liang Long, Lyu Shichao Department of Dermatology, 306th Clinical College of People′s Liberation Army, Anhui Medical University, Beijing 100101, China（Dong Q, Xia TB, Yin MM, Wang ZK, Mi X, Liu JL, Cao XJ, Liu JJ, Ge Y, Liang L , Lyu SC）; Department of Medical Cosmetology, The People′s Hospital of Wu Qing, Tianjin 301700, China（Yang F）; Department of Dermatology, The Third People′s Hospital, Nanhai District, Foshan City, Foshan 528200, Guangdong, China（Ren YY） Corresponding author: Lyu Shichao, Email: lvshichao@sina.com 【Abstract】 Objective To investigate human papillomavirus （HPV） genotypes in adult patients with plantar warts, and to explore their relationship with clinical features of adult plantar warts. Methods PCR, gene sequencing and TA-cloning technique were performed to detect HPV types in 221 patients with plantar warts. Meanwhile, clinical data were recorded, including patients′ age, disease duration, symptoms and number of warts. Results Of 221 specimens, HPV DNA was detected in 215, with the HPV-positive rate being 97%. Single HPV infections were detected in 206 specimens （96%, 206/215）, among which, HPV types included HPV-27 （44.7%, 92/206）, -2 （12.1%, 25/206）, -57 （7.3%, 15/206）, -7 （Alpha genus; 0.5%, 1/206）, -65 （Gamma genus; 0.5%, 1/206）, -1 （33.5%, 69/206） and -63 （Mu genus; 1.5%, 3/206）. There were no significant differences in age, disease duration, number of warts, incidence of pain and gender among patients with HPV-27, -2 or -57 infections. Compared with patients with HPV-27 infection, patients with HPV-1 infection were more related to age ≤ 30 years, disease duration ≤ 1 year, number of warts ≤ 2 and high incidence of pain. Conclusion HPV-27, -2, -57 and -1 are the main types in adult patients with plantar warts, and HPV types are correlated with clinical features of adult plantar warts.

Key words: Papillomaviridae, Genotyping techniques, Warts, PCR, Clinical features, TA cloning