中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (9): 673-675.

• 研究报道 • 上一篇    下一篇

普萘洛尔及异丙肾上腺素对婴儿血管瘤内皮细胞β2肾上腺素受体表达的影响

朱雅琳1,马龙2,帕丽达．阿布力孜1   

  1. 1. 新疆医科大学第一附属医院
    2. 新疆医科大学第一附属医院皮肤科
  • 收稿日期:2016-08-08 修回日期:2017-03-02 出版日期:2017-09-15 发布日期:2017-08-31
  • 通讯作者: 帕丽达．阿布力孜 E-mail:palidae@aliyun.com

In vitro effects of propranolol and isoproterenol on the of beta-2 adrenergic receptor on infantile hemangioma endothelial cells

2, 2   

  • Received:2016-08-08 Revised:2017-03-02 Online:2017-09-15 Published:2017-08-31

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摘要： 目的 探讨β2肾上腺素受体在婴儿血管瘤发病机制中的作用。方法 婴儿血管瘤内皮细胞分为普萘洛尔组及异丙肾上腺素组。普萘洛尔组再分为10、15、20 μg/ml组、空白组及二甲基亚砜（DMSO）组5组，异丙肾上腺素组再分为5、10、20 μg/ml组、空白组4组。血管瘤内皮细胞与这些组作用24 h、48 h，用酶联免疫吸附法分别测定每浓度细胞培养上清液中β2肾上腺素受体的表达。结果 作用24 h后，15 μg/ml、20 μg/ml组普萘洛尔与空白组和DMSO组比较，β2肾上腺素受体的表达下降，差异有统计学意义（均P＜0.05）。48 h后，普萘洛尔组各浓度培养的细胞，β2肾上腺素受体表达较空白组下降，差异有统计学意义（均P＜0.05）。而异丙肾上腺素组，作用24 h后，10 μg/ml、20 μg/ml组的异丙肾上腺素较空白组β2肾上腺素受体的表达增高，差异有统计学意义（均P＜0.05）。48 h后，20 μg/ml组异丙肾上腺素培养的细胞上清液中的β2肾上腺素受体表达较空白组高，差异有统计学意义（均P＜0.05）。结论 普萘洛尔对血管内皮细胞表面的β2肾上腺素受体表达的影响下调，而异丙肾上腺素作用上调。

关键词: 受体, 肾上腺素能β2

Abstract: Zhu Yalin, Ma Long, Paride Abliz Department of Dermatology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China Corresponding author: Paride Abliz, Email: palidae@aliyun.com 【Abstract】 Objective To explore the role of beta-2 adrenergic receptor in the pathogenesis of infantile hemangioma. Methods In vitro cultured infantile hemangioma endothelial cells were divided into propranolol and isoproterenol groups. The propranolol groups were further classified into 5 groups to be treated with propranolol solutions at concentrations of 10, 15, 20 μg/ml, EGM-2 medium （blank control group 1）, and EGM-2 medium containing 0.16% DMSO （DMSO group） respectively, while the isoproterenol groups were classified into 4 groups to be treated with isoproterenol solutions at concentrations of 5, 10, 20 μg/ml and EGM-2 medium （blank control group 2） respectively. After 24- and 48-hour treatment, enzyme-linked immunosorbent assay （ELISA） was performed to measure the of beta-2 adrenergic receptor in cell culture supernatants in the above groups. Results After 24-hour treatment, 15-μg/ml and 20-μg/ml propranolol groups showed significantly decreased of beta-2 adrenergic receptor compared with blank control group 1 and DMSO group （all P < 0.05）. After 48-hour treatment, all the propranolol groups showed significantly decreased of beta-2 adrenergic receptor compared with the blank control group 1（all P < 0.05）. However, the of beta-2 adrenergic receptor was significantly higher in the 10- and 20-μg/ml isoproterenol groups than in the blank control group 2 after 24-hour treatment （all P < 0.05）, and higher in the 20-μg/ml isoproterenol group than in the blank control group 2 after 48-hour treatment （P < 0.05）. Conclusion Propranolol can down-regulate the of beta-2 adrenergic receptor on the surface of vascular endothelial cells, while isoproterenol can up-regulate its .