关键词: 基因, rpoB

Abstract:

Jiang Yong, Yang Lina, Liu Yuanjun, Hou Shuping, Qi Manli, Liu Quanzhong*. *Department of Dermatology and Venereology, General Hospital of Tianjin Medical University, Tianjin 300052, China Corresponding author: Liu Quanzhong, Email：liuquanzhong@medmail.com.cn 【Abstract】 Objective To evaluate the susceptibility of Chlamydia trachomatis clinical isolates to rifampin, and assess the relationship between rpoB mutations and antibiotic resistance in them. Methods A microculture method was used to determine the minimal inhibitory concentration （MIC） of rifampin in 52 Chlamydia trachomatis clinical isolates. The rpoB gene was amplified from all the clinical isolates and a standard strain of Chlamydia trachomatis followed by single-strand conformation polymorphism （SSCP）analysis. Sequencing of PCR products was carried out for two clinical isolates. Results No rifampin-resistant strain was found among these clinical isolates. The MIC of rifampin varied from 0.004 to 0.030 mg/L. Neither SSCP analysis nor sequencing showed rpoB mutations. Conclusions No rpoB mutations were found in Chlamydia trachomatis isolates from patients unresponsive to rifampin. The unresponsiveness to rifampin may be attributed to multiple factors.