中华皮肤科杂志 ›› 2015, Vol. 48 ›› Issue (1): 15-18.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患儿Epstein-Barr病毒基因异常表达的研究

丁艳1,何小解2,廖旺3,杨慧兰4,向伟5,党西强6,易著文6   

  1. 1. 海南省妇幼保健院科教科
    2. 长沙 中南大学湘雅二医院儿童医学中心小儿肾脏专科
    3. 海南省人民医院心血管内科
    4. 广州军区总医院皮肤科
    5. 海南省妇幼保健院海南省儿童医院
    6. 长沙，中南大学湘雅二医院儿童医学中心小儿肾脏专科
  • 收稿日期:2014-08-05 修回日期:2014-09-30 发布日期:2019-06-12
  • 通讯作者: 杨慧兰 E-mail:huilany88@vip.163.com
  • 基金资助:
    海南省自然科学基金;海南省自然科学基金

Aberrant expression of Epstein-Barr virus genes in children with systemic lupus erythematosus

  • Received:2014-08-05 Revised:2014-09-30 Published:2019-06-12
  • Supported by:
    ;Natural Science Foundation of Hainan Province of China

PDF

14

摘要： 目的 探讨Epstein-Barr病毒（EBV）基因在儿童系统性红斑狼疮（SLE）中的表达和意义。 方法 分离20例SLE患儿和12例健康对照外周血单一核细胞（PBMC），用酶联免疫吸附测定（ELISA）鉴定出血清中编码EBV衣壳抗原-IgG/IgM抗体阳性的SLE患儿，取其分泌EBV的细胞上清液与提取的PBMC共同培养12 d，提取培养前后的PBMC的RNA，用反转录实时定量PCR（RT-PCR）的方法检测EBV基因的表达。 结果 10例患儿和1例健康对照检测到LMP1表达，两者差异有统计学意义（P < 0.05）。4例患儿和1例健康对照检测到LMP2表达，13例患儿和3例健康对照检测到EBNA1表达，3例患儿和1例健康对照检测到BCRF1表达，5例患儿和2例健康对照检测到BLLF1表达，但患儿和健康对照比较，差异均无统计学意义。用分泌EB病毒的细胞上清液培养PBMC后，发现患儿组和健康对照组潜伏期基因和裂解期基因表达均有不同程度的增加，潜伏期基因LMP1、LMP2、EBNA-1和裂解期基因BCRF1、BLLF1的表达与健康对照组比较，差异有统计学意义（P < 0.05）。 结论 儿童SLE患者存在EBV基因的异常表达，EBV基因可能参与了SLE的发病。

关键词: 红斑狼疮，系统性, EB病毒感染, 基因表达, 儿童

Abstract: Ding Yan*, He Xiaojie, Liao Wang, Yang Huilan, Xiang Wei, Dang Xiqiang, Yi Zhuwen. *Graduate School of Southern Medical University, Guangzhou 510015, China Corresponding authors: Yang Huilan, Email: huilany88@hotmail.com; Xiang Wei, Email: xiangwei8@163.com 【Abstract】 Objective To investigate the expression and significance of Epstein-Barr virus （EBV） genes in children with systemic lupus erythematosus （SLE）. Methods Peripheral blood mononuclear cells （PBMCs） were isolated from 20 children with SLE and 12 healthy human controls. Enzyme-linked immunosorbent assay （ELISA） was conducted to detect anti-EBV viral capsid antigen （VCA） IgG/IgM antibodies. The culture supernatants of cells from patients with anti-EBV VCA IgG/IgM antibodies were collected, and PBMCs from the patients and controls were co-cultured with the supernatants respectively for 12 days. RNA was extracted from PBMCs before and after the coculture, and reverse transcription-PCR was performed to detect the expression of EBV genes, including LMP1, LMP2, EBNA1, BCRF1, BLLF1 and BILF1 genes. Results LMP1 gene was detected in fresh PBMCs from 10 out of 20 patients and 1 out of 12 controls （P < 0.05）. No significant differences were observed between the patients and controls in the detection rate of LMP2 gene （4/20 vs. 1/12）, EBNA1 gene （13/20 vs. 3/12）, BCRF1 gene （3/20 vs. 1/12） or BLLF1 gene （5/20 vs. 2/12） in fresh PBMCs. After co-culture with the supernatants of cells from patients with anti-EBV VCA IgG/IgM antibodies, the expressions of EBV genes in these PBMCs were increased to different degrees, and there was a significant difference in the expressions of EBV latent genes LMP1, LMP2 and EBNA-1 as well as EBV replicative genes BCRF1 and BLLF1 between the patient-derived and control-derived PBMCs （all P < 0.05）. Conclusions There is an aberrant expression of EBV genes in children with SLE, and EBV genes may contribute to the development of SLE.

Key words: Lupus erythematosus, systemic, Epstein-Barr virus infections, Gene expression, Child

引用本文

丁艳 何小解 廖旺 杨慧兰 向伟 党西强 易著文. 系统性红斑狼疮患儿Epstein-Barr病毒基因异常表达的研究[J]. 中华皮肤科杂志, 2015,48(1):15-18. doi: