中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (10): 728-731.

• 论著 • 上一篇    下一篇

阿魏酸对黑素细胞增殖、黑素合成、酪氨酸酶活性及c-kit、ERK蛋白表达的影响

吴艳华1,汤楠1,蔡兰花1,李其林2   

  1. 1. 暨南大学第四附属医院（广州红十字会医院）中医科
    2. 暨南大学第四附属医院，广州市红十字会医院皮肤科
  • 收稿日期:2014-07-04 修回日期:2014-07-07 发布日期:2014-10-01
  • 通讯作者: 吴艳华 E-mail:wuyanhua368@163.com
  • 基金资助:
    广东省科技计划项目

Effect of ferulic acid on the proliferation of, as well as melanin synthesis, tyrosinase activity and expressions of c-kit and ERK proteins in keratinocytes

  • Received:2014-07-04 Revised:2014-07-07 Published:2014-10-01

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摘要： 【摘要】 目的 探讨阿魏酸对体外培养的正常人表皮黑素细胞增殖、黑素合成、酪氨酸酶活性及c-kit、ERK蛋白表达的影响。 方法 以不同浓度的阿魏酸干预体外培养的正常人表皮黑素细胞，用MTS法分别检测培养24、48、72 h后黑素细胞的增殖活性。用NaOH裂解法检测培养72 h后黑素细胞的黑素合成。用多巴氧化反应法测定培养72 h后黑素细胞酪氨酸酶活性。用Western印迹法测定培养72 h黑素细胞c-kit蛋白及ERK1/2蛋白表达水平。 结果 与对照组相比，0.01、0.1、1 mg/ml阿魏酸在作用24、48、72 h后，抑制黑素细胞增殖作用的差异有统计学意义（均P ＜ 0.05），其中1 mg/ml阿魏酸作用72 h抑制黑素细胞活性最高。0.01、0.1、1 mg/ml阿魏酸均能显著影响黑素合成和酪氨酸酶活性，并可降低黑素细胞中c-kit蛋白及ERK1/2蛋白表达水平，与对照组相比，差异有统计学意义（均P ＜ 0.05）。结论 阿魏酸可抑制培养的人表皮黑素细胞增殖、黑素合成及酪氨酸酶活性，并可下调黑素细胞c-kit蛋白及ERK蛋白的表达。

关键词: 阿魏酸, 黑素细胞, 细胞增殖, 酪氨酸, 原癌基因蛋白质c-kit

Abstract: Wu Yanhua*, Tang Nan, Cai Lanhua, Li Qilin. *Fourth Affiliated Hospital of Jinan University School of Medicine; Department of Traditional Chinese Medicine, Guangzhou Red Cross Hospital, Guangzhou 510220, China Corresponding author: Wu Yanhua, Email: wuyanhua368@163.com 【Abstract】 Objective To evaluate the in vitro effect of ferulic acid on the proliferation of, as well as melanin synthesis, tyrosinase activity and expressions of c-kit and ERK proteins in cultured normal human epidermal melanocytes. Methods Cultured normal human epidermal melanocytes were treated with various concentrations of ferulic acid for different durations, and those remaining untreated served as the control. Then, 3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium, inner salt （MTS） assay was performed to estimate cell proliferative activity at 24, 48 and 72 hours, sodium hydroxide solubilization method to quantify melanin content in melanocytes at 72 hours, dopa oxidation assay to evaluate tyrosinase activity at 72 hours, Western blot to measure the expressions of c-kit and ERK1/2 proteins at 72 hours. Results Cellular proliferative activity was significantly inhibited in melanocytes treated with ferulic acid of 0.01, 0.1 and 1 mg/ml for 24, 48 and 72 hours compared with untreated melanocytes （all P < 0.05）, and the 72-hour treatment with ferulic acid of 1 mg/ml showed the strongest inhibitory effect. Ferulic acid at 0.01, 0.1 and 1 mg/ml all markedly suppressed melanin synthesis and tyrosinase activity, decreased the expressions of c-kit and ERK1/2 proteins in melanocytes, with significant differences in these parameters between ferulic acid-treated and untreated melanocytes （all P < 0.05）. Conclusions Ferulic acid could downregulate the proliferation of, as well as melanin synthesis, tyrosinase activity, and expressions of c-kit and ERK proteins in cultured human epidermal melanocytes.

Key words: Ferulic acid, Melanocytes, Cell proliferation, Tyrosine, Proto-oncogene proteins c-kit

引用本文

吴艳华 汤楠 蔡兰花 李其林. 阿魏酸对黑素细胞增殖、黑素合成、酪氨酸酶活性及c-kit、ERK蛋白表达的影响[J]. 中华皮肤科杂志, 2014,47(10):728-731. doi: