中华皮肤科杂志

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银杏石榴煎对寻常性银屑病患者血小板活化因子及其受体表达水平的影响

羌建峰, 曹双林   

  1. 南通大学附属医院皮肤性病科, 江苏省, 226001
  • 收稿日期:2006-11-22 出版日期:2007-10-15 发布日期:2007-10-15
  • 通讯作者: 曹双林,email:slcaol@yahoo.com.cn E-mail:slcaol@yahoo.com.cn

Effects of Yinxing Shiliu Jian on the level of platelet activating factor(PAF),and expression of PAF-receptor(PAFR)in patients with psoriasis vulgaris

QIANG Jian-feng, CAO Shuang-lin   

  1. Department of Dermatology, Affiliated Hospital of Nantong Medical University, Nantong 226001, Jiangsu, China
  • Received:2006-11-22 Online:2007-10-15 Published:2007-10-15

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摘要： 目的 探讨中药银杏石榴煎治疗进行期寻常性银屑病的疗效及其对患者血清中血小板活化因子(PAF)及皮损组织中血小板活化因子受体(PAFR)表达水平的影响。方法 治疗前后对银屑病患者皮损进行PASI评分.采用ELISA法检测患者治疗前后和正常人对照血清中PAF的表达水平,并采用免疫组化方法检测患者治疗前后皮损和正常人皮肤中PAFR的表达变化。结果 ①银杏石榴煎可显著改善患者的临床症状,治疗后(2.86±1.81)PASI评分较治疗前(32.81±14.77)明显下降(P<0.01);②治疗前患者血清中PAF表达水平(15.41±4.28 pg/mL)明显高于正常人对照(5.77±1.47 pg/mL)(P<0.01),治疗后(8.25±2.43 pg/mL)其表达水平较治疗前明显下降(P<0.05),且患者血清中PAF表达水平与PASI评分呈正相关(r=0.46,P<0.05);③治疗前患者皮损表皮及真皮中内皮细胞、炎症细胞可见明显PAFR阳性表达,治疗后阳性细胞表达减少(P<0.05)。结论 进行期银屑病患者血清中PAF及皮损中PAFR呈高表达,且与患者病情严重程度密切相关,银杏石榴煎可明显降低它们的表达水平。

关键词: 银屑病, 中草药, 血小板活化因子, 血小板活化因子受体

Abstract: Objective To evaluate the efficacy of Yinxing Shiliu Jian in the treatment of psoriasis vulgaris(PV)and its effects on the level of PAF in sera,and the expression of PAFR in lesions of patients with PV.Methods Forty-six patients with PV enrolled into this study.The patients received oral Yinxing Shiliu Jian thrice a day for 1 to 3 months(1 month as a treatment session);changes in the clinical signs of the disease were evaluated at the end of every treatment session using the psoriasis area severity index (PASI)score.ELISA was used to measure the serum levels of PAF in all patients before treatment,15 patients after treatment,and 10 normal human controls.The expression of PAFR was detected by immuno-histochemical method in typical active lesions of 20 patients before the start of the medication,in subsiding lesions of 8 patients after medication,and in 5 foreskin specimens to serve as control.Results The PASI scores of these patients significantly decreased after treatment with the Chinese medicine(2.858±1.813 vs 32.814±14.768,P<0.01).The serum level of PAF in patients before treatment was significantly higher than that in normal human controls(15.407±4.277 pg/mL vs 5.769±1.469 pg/mL,P<0.01),and in patients after treatment(15.407±4.277 pg/mL vs 8.251±2.432 pg/mL,P<0.05).Furthermore,the level of PAF was positively correlated with PASI score in PV patients(r=0.46,P<0.05).The numbers of PAFR-positive cells were significantly lower in angio-endotbelial cells and inflammatory cells in lesional epidermis and dermis after treatment compared to the corresponding numbers before treatment(P<0.05).Conclusions There is a high expression of PAF in sera,and PAFR in lesions of progressive PV patients.The level of PAF and the number of PAFR-positive ceils closely correlated with the disease severity;the Yinxing Shiliu Jian could clearly downregulate the high level of PAF,and the high expression of PAFR.

Key words: Psoriasis, Drugs, Chinese herbal, Platelet activating factor, Receptor, platelet activating factor