中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (1): 10-12.

• 论著 • 上一篇    下一篇

BP180NC16a-ELISA对大疱性类天疱疮血清学诊断的评价

冯素英, 吴勤学, 林麟, 靳培英, 周武庆, 桑红桂   

  1. 中国医学科学院、中国协和医科大学皮肤病医院 南京 210042
  • 收稿日期:2006-01-16 出版日期:2007-01-15 发布日期:2007-01-15

Evaluation of a BP180-NC16a enzyme-linked immunosorbent assay in the serodiagnosis of bullous pemphigoid

FENG Su-ying, WU Qin-xue, LIN Lin, JIN Pei-ying, ZHOU Wu-qing, SANG Hong-gui   

  1. Intitute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042, China
  • Received:2006-01-16 Online:2007-01-15 Published:2007-01-15

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摘要： 目的 评价BP180NC16a-ELISA对大疱性类天疱疮血清学诊断的效能.方法 BP患者42例,对照组42例(其中正常人对照24例,天疱疮18例),在患者用药前采血,比较BP180NC16a-ELISA和盐裂试验免疫荧光(IIF)检测的结果.结果 用BP180NC16a-ELISA检测时,BP患者中有1例呈阴性反应,其敏感性为97.62%;正常人对照组中有1例呈阳性反应,其特异性为97.62%,且BP180NC16a-ELISA法的A值与IIF滴度之间无相关性.结论 BP180NC16a-ELISA在疾病初始阶段是检测血清中抗BP180抗体的有效方法.

关键词: HIF-1α, VEGF, Ang-2, 妊娠, 尖锐湿疣, 类天疱疮, 大疱性, 诊断, BP180, ELISA

Abstract: Objective To evaluate the efficacy of a BP180NC16a-ELISA in the initial serodiagnosis of bullous pemphigoid(BP). Methods Sera from BP patients(n-42)and control subjects (healthy controls, n=24; pemphigus, n=18)were assayed by BP180-NC16a ELISA. All BP sera were obtained at presentation before initiation of systemic immunosuppressive therapy. The values of IgG antibody levels measured by ELISA were compared with those measured by indirect IF on salt-split skin. Results The sensitivity and specificity of the ELISA kit were 97.62%(41 of 42 BP sera showed a positive result) and 97.62%(23 of 24 normal controls and all the pemphigus sera showed a negative result),respectively. There was no correlation between the mean ELISA values and indirect IF titers. Conclusion The results suggest that the BP180-NCl6a ELISA is a useful tool for the detection of pathogenic anti-BP180 IgG autoantibodies at the initial disease stage of BP.

Key words: Pemphigoid, bullous, Diagnosis, BP180, ELISA