中华皮肤科杂志 ›› 2026, Vol. 59 ›› Issue (2): 161-166.doi: 10.35541/cjd.20250389

• 研究报道 • 上一篇    下一篇

蕈样肉芽肿患者36例皮损组织中CD103的分布和表达研究

黎钊1    金莎2    赵一格1    王佳绮1    王平1   

  1. 1杭州市第三人民医院皮肤科，杭州  310009 ；2浙江中医药大学附属杭州市第三人民医院皮肤科，杭州  310009
  • 收稿日期:2025-07-11 修回日期:2025-12-12 发布日期:2026-02-03
  • 通讯作者: 王平 E-mail:dermwang@aliyun.com
  • 基金资助:
    浙江省基础公益研究计划（LBZ22H160001）

Distribution and expression of CD103 in skin lesions of 36 patients with mycosis fungoides

Li Zhao1, Jin Sha2, Zhao Yige1, Wang Jiaqi1, Wang Ping1   

  1. 1Department of Dermatology, Hangzhou Third People's Hospital, Hangzhou 310009, China; 2Department of Dermatology, Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou 310009, China
  • Received:2025-07-11 Revised:2025-12-12 Published:2026-02-03
  • Contact: Wang Ping E-mail:dermwang@aliyun.com
  • Supported by:
    Zhejiang Provincial Basic Public Welfare Research Program（LBZ22H160001）

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摘要： 【摘要】 目的 探讨CD103在不同临床分期及病理亚型蕈样肉芽肿（MF）中的分布和表达情况。方法 本研究为回顾性病例系列研究。分析2017年1月至2021年1月在杭州市第三人民医院皮肤科门诊就诊的36例MF患者的临床病理资料。对2例肿瘤期MF患者的皮肤活检组织进行单细胞RNA测序。应用免疫组化法检测36例MF患者皮损中CD103和CD45RO的表达。呈正态分布的数据，多组比较用单因素方差分析，多重比较采用Tukey检验。结果 36例MF患者中，经典型MF 20例（斑片期9例、斑块期8例、肿瘤期3例），男10例，女10例，年龄（48.90 ± 17.51）岁；嗜毛囊性MF（FMF）8例，男4例，女4例，年龄（35.13 ± 24.54）岁；色素减退性MF（HMF）8例，均为男性，年龄（14.50 ± 9.41）岁。单细胞RNA测序并聚类后得到16个T细胞亚群（C01至C16），其中C01、C03、C11、C13四个细胞簇具有高拷贝数变异特征，提示为恶性T细胞，这4个细胞簇均表现为CD103 mRNA特异性高表达。皮损免疫组化检测：经典型MF的斑片期，CD103+组织驻留记忆T细胞（TRM）特征性沿表皮基底层呈线状排列；在斑块期，CD103+ TRM呈现明显亲表皮现象，并可见Pautrier微脓肿；在肿瘤期，CD103+ TRM则失去亲表皮性，于真表皮中散在分布。HMF病例CD103染色均阴性。FMF病例中CD103+ TRM主要浸润毛囊及真皮小血管周围。经典型MF斑片期、斑块期、肿瘤期患者CD103+ TRM细胞计数（5个高倍视野中细胞数）差异无统计学意义（F = 3.06，P = 0.073）。HMF组、经典型MF组、FMF组间CD103+ TRM细胞计数差异有统计学意义（HMF组为8.91 ± 6.82，经典型MF组为124.13 ± 122.39，FMF组为110.24 ± 109.15；F = 3.50，P = 0.042），HMF组低于经典型MF组（P < 0.001）和FMF组（P = 0.005）。CD103/CD45RO比值在经典型MF不同分期组间差异有统计学意义（斑片期为0.12 ± 0.08，斑块期为0.41 ± 0.20，肿瘤期为0.30 ± 0.35；F = 5.24，P = 0.017），斑片期比值小于斑块期（P = 0.013）；CD103/CD45RO比值在不同亚型组间差异亦有统计学意义（HMF组为0.03 ± 0.02，经典型组为0.26 ± 0.22，FMF组为0.34 ± 0.21；F = 5.87，P = 0.007），HMF组比值低于经典型MF组（P = 0.002）和FMF组（P < 0.001）。结论 CD103的表达特征有助于MF的早期识别与亚型鉴别，可作为辅助诊断与评估疾病阶段的潜在分子标志物。

关键词: 蕈样真菌病, CD103, CD45RO, 组织常驻记忆性T细胞, 病理学, 分子, 免疫组织化学

Abstract: 【Abstract】 Objective To investigate the distribution and expression of CD103 in skin lesions of mycosis fungoides （MF） across different clinical stages and pathological subtypes. Methods This retrospective case series study analyzed the clinical and pathological data from 36 patients with MF who visited the Department of Dermatology, Hangzhou Third People's Hospital from January 2017 to January 2021. Single-cell RNA sequencing was performed on skin biopsy samples from 2 patients with tumor-stage MF. Immunohistochemical study was conducted to assess the expression of CD103 and CD45RO in skin lesions of all the 36 patients. Normally distributed data were compared among multiple groups using one-way analysis of variance, followed by Tukey′s test for multiple comparisons. Results Among the 36 MF patients, there were 20 with classic MF （9 in the patch stage, 8 in the plaque stage, 3 in the tumor stage）, including 10 males and 10 females, aged 48.90 ± 17.51 years; 8 patients had folliculotropic MF （FMF）, including 4 males and 4 females, aged 35.13 ± 24.54 years; 8 had hypopigmented MF （HMF）, all of whom were males, aged 14.50 ± 9.41 years. Single-cell RNA sequencing and clustering analysis identified 16 T cell subsets （C01 - C16）, among which, 4 clusters （C01, C03, C11, C13） exhibited high copy number variation, indicating their malignant nature, and all the 4 clusters showed specifically high CD103 mRNA expression. Immunohistochemical study showed that CD103? tissue-resident memory T （TRM） cells were characteristically arranged linearly along the basal layer of the epidermis in patch-stage classic MF; in plaque-stage MF, CD103? TRM cells showed prominent epidermotropism with the presence of Pautrier microabscesses; in tumor-stage MF, CD103? TRM cells lost epidermotropism, and were scattered in the dermis and epidermis. All HMF cases were negative for CD103 staining. In FMF cases, CD103? TRM cells mainly infiltrated around hair follicles and small blood vessels in the dermis. The number of CD103? TRM cells （counted in 5 high-power fields） did not significantly differ among patients with patch, plaque, and tumor stages of classic MF （F = 3.06, P = 0.073）. However, CD103? TRM counts significantly differed among HMF, classic MF, and FMF groups （HMF: 8.91 ± 6.82; classic MF: 124.13 ± 122.39; FMF: 110.24 ± 109.15; F = 3.50, P = 0.042）, with the HMF group showing significantly lower counts compared with the classic MF group （P < 0.001） and FMF group （P = 0.005）. The CD103/CD45RO ratio varied significantly among different stages of classic MF （patch stage: 0.12 ± 0.08; plaque stage: 0.41 ± 0.20; tumor stage: 0.30 ± 0.35; F = 5.24, P = 0.017）, with a lower ratio in the patch stage than in the plaque stage （P = 0.013）; significant differences were also observed in the CD103/CD45RO ratio among different MF subtypes （HMF: 0.03 ± 0.02; classic MF: 0.26 ± 0.22; FMF: 0.34 ± 0.21; F = 5.87, P = 0.007）, which was significantly lower in the HMF group than in the classic MF group （P = 0.002） and FMF group （P < 0.001）. Conclusion The expression profile of CD103 facilitates the early diagnosis and subtype differentiation of MF, and may serve as a potential molecular marker for auxiliary diagnosis and disease stage assessment.

Key words: Mycosis fungoides, CD103, CD45RO, Tissue resident memory T cell, Pathology, molecular, Immunohistochemistry

引用本文

黎钊 金莎 赵一格 王佳绮 王平. 蕈样肉芽肿患者36例皮损组织中CD103的分布和表达研究[J]. 中华皮肤科杂志, 2026,59(2):161-166. doi:10.35541/cjd.20250389

Li Zhao, Jin Sha, Zhao Yige, Wang Jiaqi, Wang Ping. Distribution and expression of CD103 in skin lesions of 36 patients with mycosis fungoides[J]. Chinese Journal of Dermatology, 2026, 59(2): 161-166.doi:10.35541/cjd.20250389