中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (7): 490-493.

• 论著 • 上一篇    下一篇

转化生长因子β对A431细胞上皮向间质转换相关分子表达的影响

郭圆圆1,彭斌1,向桂琼2,耿松梅1   

  1. 1. 西安交通大学第二附属医院皮肤科
    2. 西安交通大学医学院第二附属医院皮肤科
  • 收稿日期:2013-09-04 修回日期:2014-02-26 发布日期:2014-07-01
  • 通讯作者: 耿松梅 E-mail:gsm312@yahoo.com
  • 基金资助:
    教育部新世纪人才支持计划;西安交通大学第二附属医院基金;国家自然科学基金面上项目

Effects of transforming growth factor-β on the expressions of epithelial-to-mesenchymal transition-related molecules in A431 cells

  • Received:2013-09-04 Revised:2014-02-26 Published:2014-07-01

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摘要： 目的 研究转化生长因子β（TGF-β）对人表皮鳞状细胞癌A431细胞上皮向间质转换（EMT）相关分子表达的影响。 方法 用含7.5 μg/L TGF-β处理A431细胞72 h后观察细胞形态，采用实时定量PCR和Western印迹分别在mRNA水平和蛋白水平检测EMT相关基因E钙黏着蛋白、N钙黏着蛋白、波形蛋白、β联蛋白表达水平的变化。未经TGF-β处理的A431细胞为对照组。 结果 TGF-β处理后，A431细胞独立分散，呈梭形。实时定量PCR结果显示，E钙黏着蛋白表达量为未经TGF-β处理组的31.7%，N钙黏着蛋白、波形蛋白、β联蛋白表达量分别为未经处理组的2.475、11.340、2.615倍；Western印迹结果，N钙黏着蛋白、波形蛋白、β联蛋白表达量上升，E钙黏着蛋白表达量下降。 结论 TGF-β可诱导A431细胞产生EMT现象，TGF-β表达量上升，可能参与表皮鳞状细胞癌发生侵袭、转移。

关键词: 细胞系，肿瘤, 转化生长因子β, 上皮向间质转化

Abstract: Guo Yuanyuan, Peng Bin, Xiang Guiqiong, Geng Songmei. Department of Dermatology, Second Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710004, China Corresponding author: Geng Songmei, Email: gsm312@yahoo.com 【Abstract】 Objective To evaluate the effect of transforming growth factor-β （TGF-β） on the expressions of epithelial-to-mesenchymal transition （EMT）-related molecules in human A431 epidermal squamous cell carcinoma cells. Methods Cultured A431 cells were classified into two groups: TGF-β group treated with TGF-β of 7.5 μg/L for 72 hours, and control group remaining untreated. Subsequently, cell morphology was observed under an inverted microscope, and real time-PCR and Western blot were performed to quantify the mRNA and protein expressions of EMT-related molecules including E-cadherin, N-cadherin, vimentin and β-catenin respectively. Results After TGF-β treatment, the cells became dispersed and spindle in shape. The mRNA expression levels of E-cadherin, N-cadherin, vimentin, and β-catenin in TGF-β group were 0.317, 2.475, 11.340 and 2.615 folds those in the control group respectively. Western blot showed a marked increase in the expression of N-cadherin, vimentin, and β-catenin proteins but a notable decrease in that of E-cadherin in the TGF-β group compared with the control group. Conclusions TGF-β can induce EMT in A431 cells, and increased expression of TGF-β may contribute to the invasion and metastasis of SCC.

Key words: Cell line, tumor, Transforming growth factor beta, Epithelial-to-mesenchymal transition

引用本文

郭圆圆 彭斌 向桂琼 耿松梅. 转化生长因子β对A431细胞上皮向间质转换相关分子表达的影响[J]. 中华皮肤科杂志, 2014,47(7):490-493. doi: