关键词: 抗层黏连蛋白γ1类天疱疮, 抗P200类天疱疮, 印迹法，蛋白质

Abstract: Li Zhiliang, Zhang Xiaodong, Chen Hao, Liu Musang, Jin Peiying, Feng Suying, Wang Baoxi. Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs; Hospital of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China Corresponding authors: Wang Baoxi, Email: wangbx@ncstdlc.org; Feng Suying, Email: fengsuying2010@aliyun.com 【Abstract】 A 55-year-old male patient presented with tense bullae on the extremities and trunk. Histological examination revealed subepidermal vesicles and superficial dermal infiltration of eosinophils and lymphocytes. The patient was primarily diagnosed with bullous pemphigoid. However, serum autoantibodies of the patient bound to the dermal side of salt-split skin, and no serum antibodies against BP180, BP230 or type Ⅶ collagen were detected by enzyme-linked immunosorbent assay. Hence, the diagnoses of bullous pemphigoid and epidermolysis bullosa acquisita were excluded. As Western blot and immunoprecipitation analysis showed, there existed antibodies capable of binding to a dermal antigen with a relative molecular mass of 200 000 in the serum of the patient. Based on the above findings, the patient was diagnosed as anti-laminin γ1 （p200） pemphigoid.

Key words: Anti-laminin γ1 pemphigoid, Anti-p200 pemphigoid, Blotting, Western