地塞米松对系统性红斑狼疮阴虚内热证患者外周血单一核细胞基因表达谱的影响

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (4): 280-282.

地塞米松对系统性红斑狼疮阴虚内热证患者外周血单一核细胞基因表达谱的影响

程喜平¹,范瑞强²,赖梅生³,陈达灿⁴,禤国维³,朱晓浚⁵,陈明春⁶

1. 广州医学院第一附属医院皮肤科
2. 广州市广东省中医院皮肤科
3. 广州广东省中医院皮肤科
4. 广州广东省中医院皮肤科
5. 中山大学附属第二医院皮肤科
6. 广州，中山大学附属第二医院皮肤科

收稿日期:2010-06-01 修回日期:2010-12-15 出版日期:2011-04-15 发布日期:2011-04-01
通讯作者: 程喜平 E-mail:cxpgz@126.com
基金资助:
国家自然科学基金滋阴清热方影响阴虚内热证SLE白细胞基因及蛋白质表达模式的动态研究

Influence of dexamethasone on the gene expression profiling of peripheral blood mononuclear cells （PBMCs） from patients with internal heat due to Yin deficiency-type SLE

Received:2010-06-01 Revised:2010-12-15 Online:2011-04-15 Published:2011-04-01

摘要/Abstract

摘要：

目的探讨地塞米松对系统性红斑狼疮（SLE）阴虚内热证患者外周血单一核细胞（PBMC）基因表达谱的影响。方法用地塞米松孵化SLE阴虚内热证患者PBMC，收集孵化后细胞进行PBMC的基因表达谱检测。结果地塞米松上调85个PBMC基因，下调126个PBMC基因。结论地塞米松调控SLE阴虚内热证患者PBMC的基因所属功能簇众多，基因编码的功能蛋白分布细胞多个部位，不是所有基因调控结果均有利于SLE缓解，一些基因调控作用对研究地塞米松治疗SLE的机制有帮助。

关键词: 基因芯片

Abstract:

Objective To observe the influence of dexamethasone on the gene expression profiling of PBMCs from patients with internal heat due to Yin deficiency-type SLE. Methods The PBMCs from 3 patients with internal heat due to Yin deficiency-type SLE were incubated with dexamethasone of 10-6 g/L for 48 hours followed by the detection of gene expression profiling of PBMCs by a GeneChip. Results The incubation with dexamethasone upregulated the expressions of 85 genes and downregulated those of 126 genes in PBMCs from the SLE patients. Conclusions Dexamethasone can regulate the expressions of numerous clusters of genes in PBMCs from patients with internal heat due to Yin deficiency-type SLE, and functional proteins encoded by these genes are located at various organelles and regions of cells. Not all the results of gene regulation by dexame-thasone favor the relief of SLE. To study the regulation of genes may be beneficial to the elucidation of mechanisms underlying the therapeutic effect of dexamethasone on SLE.

Key words: Genechip

程喜平范瑞强赖梅生陈达灿禤国维朱晓浚陈明春. 地塞米松对系统性红斑狼疮阴虚内热证患者外周血单一核细胞基因表达谱的影响[J]. 中华皮肤科杂志, 2011, 44(4): 280-282.

参考文献

Cheng R Y S, Birely L A , et al. Lum N L Expressions of hepatic genes, especially IGF-binding protein-1, correlating with serum corticosterone in microarray analysis. J Mol Endocrinol 2004;32(1):257–278 Wang JC, Derynck MK, Nonaka DF, et al. Chromatin immunoprecipitation (ChIP) scanning identifies primary glucocorticoid receptor target genes. Proc Natl Acad Sci U S A. 2004 ;101(44):15603-15608. Gladkevich A, Nelemans SA, et al. Kauffman HF, Microarray profiling of lymphocytes in internal diseases with an altered immune response: potential and methodology Mediators Inflamm. 2005(6):317-330 程喜平，范瑞强，许德清 SLE基因芯片表达谱研究进展.国际皮肤性病学杂志.2006，32（6）：346－348 吴元胜; 范瑞强; 陈达灿; et al.基因芯片技术分析系统性红斑狼疮患者外周血白细胞基因表达谱的初步研究第一军医大学学报 2005 25(8)929-934 韩光明; 陈顺乐; 沈南; et al.系统性红斑狼疮患者及其家庭成员外周血基因表达谱的研究中华皮肤科杂志2004 37(1)8-11 Esposito P, Balletta MM, Procino A, et al. Interleukin-6 release from peripheral mononuclear cells is associated to disease activity and treatment response in patients with lupus nephritis. Lupus. 2009;18(14):1329-1330. Harigai M, Kawamoto M, Hara M, et al. Excessive production of IFN-gamma in patients with systemic lupus erythematosus and its contribution to induction of B lymphocyte stimulator/B cell-activating factor/TNF ligand superfamily-13B J Immunol. 2008;181(3):2211-2219. Tsukamoto H, Horiuchi T. Clinical aspects of the complement system Rinsho Byori. 2006 ; 54(7) :757-62 Jia EZ, Wang J, Yang ZJ, et al. Association of the mutation for the human carboxypeptidase E gene exon 4 with the severity of coronary artery atherosclerosis.Mol Biol Rep. 2009;36(2):245-254. Association of the mutation for the human carboxypeptidase E gene exon 4 with the severity of coronary artery atherosclerosis. Herberg JA, Beck S, Trowsdale J et al. TAPASIN, DAXX, RGL2, HKE2 and four new genes (BING 1, 3 to 5) form a dense cluster at the centromeric end of the MHC..J Mol Biol. 1998; 277(4): 839-857. Nakamura T. Clinical strategies for amyloid A amyloidosis secondary to rheumatoid arthritis Mod Rheumatol. 2008;18(2):109-118. Vambutas A, DeVoti J, Goldofsky E, Alternate splicing of interleukin-1 receptor type II (IL1R2) in vitro correlates with clinical glucocorticoid responsiveness in patients with AIED. PLoS One. 2009;4(4):e5293. Mamtani M, Rovin B, Brey R, et al. Ann CCL3L1 gene-containing segmental duplications and polymorphisms in CCR5 affect risk of systemic lupus erythaematosus.Rheum Dis. 2008 Aug;67(8):1076-1083. Ptacek T, Li X, Kelley JM, et al. Copy number variants in genetic susceptibility and severity of systemic lupus erythematosus.Cytogenet Genome Res. 2008;123(1-4):142-147. Wang JC, Derynck MK, Nonaka DF, et al. Chromatin immunoprecipitation scanning identifies primary glucocorticoid receptortarget genes. Proc Natl Acad Sci U S A, 2004, 101: 15603-15608.

[1]	刘婷婷杨发灯林志淼汪慧君胡凌寒钟伟龙杨勇. 先天性常染色体隐性遗传性鱼鳞病两家系ABCA12基因突变分析[J]. 中华皮肤科杂志, 2018, 51(10): 737-740.
[2]	徐晓光张红顾军. 雷公藤多苷对自身免疫性甲状腺炎大鼠模型外周血CXCR3、CCR4基因表达的影响[J]. 中华皮肤科杂志, 2010, 43(11): 792-795.
[3]	佘晓东沈永年韩峻松刘泽虎吕雪莲肖华胜刘维达. Oligo基因芯片和小鼠模型研究外阴阴道念珠菌病的发病机制[J]. 中华皮肤科杂志, 2009, 42(10): 681-684.
[4]	赖维,龚子鉴,黄朝伟,黄宇青,张玉清,陈荣章,谢小元. 抑制性消减杂交结合基因芯片研究淋球菌耐头孢曲松的分子机制[J]. 中华皮肤科杂志, 2008, 41(5): 288-.