外用茶油对变应性接触性皮炎发生的影响

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (4): 282-284.

外用茶油对变应性接触性皮炎发生的影响

林朝悦¹,程波²

1. 莆田学院附属医院皮肤科
2. 福州市福建医科大学附属第一医院皮肤科(联系人苏惠春）

收稿日期:2010-05-25 修回日期:2010-12-07 出版日期:2011-04-15 发布日期:2011-04-01
通讯作者: 程波 E-mail:chengbo630415@126.com

Effects of topical application of camellia oil on the development of allergic contact dermatitis

Received:2010-05-25 Revised:2010-12-07 Online:2011-04-15 Published:2011-04-01

摘要/Abstract

摘要：

目的观察茶油对小鼠变应性接触性皮炎发生的影响。方法建立二硝基氟苯致小鼠变应性接触性皮炎模型，外用药物进行治疗，观察发生皮炎的小鼠耳片的组织学变化（HE染色），RT-PCR检测组织中炎症细胞因子IL-2、IFN-γ mRNA的表达水平。结果成功建立二硝基氟苯致小鼠变应性接触性皮炎的动物模型，且皮肤中IL-2、IFN-γ mRNA的表达显著增高。哈西奈德溶液组和茶油组的耳片组织肿胀明显减轻，炎症反应轻微，炎性细胞浸润数目少，组织病理变化轻。在茶油和哈西奈德溶液的干预下，炎性组织IL-2、IFN-γ的mRNA表达均不同程度下降。结论茶油减轻二硝基氟苯致小鼠变应性接触性皮炎的病理损害，下调了炎症因子的表达。

关键词: 变应性接触性皮炎

Abstract:

Objective To observe the effects of camellia oil on the development of allergic contact dermatitis in mice. Methods Mice models of allergic contact dermatitis were established with dinitrofluorobene （DNFB）. Then, certain concentrations of camellia oil and halcinonide were used topically on the ears of mouse models twice daily for 6 days. Skin specimens were obtained from the treated ears and subjected to histology examination and pathological analysis. RT-PCR was performed to detect the mRNA expressions of interleukin 2 （IL-2） and interferon γ （IFN-γ） in the skin tissues. Results Allergic contact dermatitis was successfully induced by DNFB in mice with a marked increase in the mRNA expression of IL-2 and IFN-γ. There was a slight decrease in the ear swelling degree, inflammatory reaction intensity, number of infiltrating inflammatory cells, and pathological severity in camellia oil-treated mice and halcinonide-treated mice compared with glycerine-treated mice. Camellia oil and halcinonide also induced different degrees of reduction in the mRNA expression of IL-2 and IFN-γ in inflammatory tissues. Conclusion Camellia oil can alleviate the pathological damage and down-regulate the expression of IL-2 and IFN-γ in mice with DNFB-induced allergic contact dermatitis.

Key words: allergic contact dermatitis

林朝悦程波. 外用茶油对变应性接触性皮炎发生的影响[J]. 中华皮肤科杂志, 2011, 44(4): 282-284.

参考文献

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