白癜风患者外周血白介素17和孤独核受体的表达

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (4): 278-279.

白癜风患者外周血白介素17和孤独核受体的表达

梁津宁,王秀敏

山东省滨州医学院附属医院皮肤科

收稿日期:2010-09-20 修回日期:2010-10-25 出版日期:2011-04-15 发布日期:2011-04-01
通讯作者: 王秀敏 E-mail:wangxiumin@medmail.com.cn

Expressions of interleukin 17 （IL-17） and RORγt in peripheral blood of patients with vitiligo

Received:2010-09-20 Revised:2010-10-25 Online:2011-04-15 Published:2011-04-01

摘要/Abstract

摘要：

目的探讨Th17细胞在白癜风免疫发病机制中的作用。方法采用双抗体夹心ELISA法检测白癜风患者进展期、稳定期和正常人外周血血清中白介素17（IL-17）水平；采用实时荧光定量RT-PCR方法检测外周血单一核细胞中孤独核受体（RORγt） mRNA表达水平，并分析其与疾病活动的相关性。结果进展期与稳定期白癜风外周血中IL-17、RORγt表达水平与正常人对照组相比显著升高（P < 0.01）。结论 Th17细胞可能与白癜风的发病机制密切相关。

关键词: RORγt

Abstract:

Objective To investigate the roles of Th17 cells in the immunological pathogenesis of vitiligo. Methods Forty patients with progressive or stable vitiligo and 20 normal human controls were included in this study. Enzyme-linked immunosorbent assay （ELISA） was performed to measure the level of serum IL-17, and SYBR Green I real-time RT-PCR to detect the mRNA expression of RORγt in peripheral blood mononuclear cells （PBMCs） from these patients. The correlation of the above parameters with disease activity was assessed. Results The levels of serum IL-17 and RORγt mRNA in PBMCs were significantly higher in patients with progressive and stable vitiligo than in normal human controls（all P<0.01）. Conclusion Th17 cells may be closely associated with the pathogenesis of vitiligo.

Key words: RORγt

梁津宁王秀敏. 白癜风患者外周血白介素17和孤独核受体的表达[J]. 中华皮肤科杂志, 2011, 44(4): 278-279.

参考文献

[1]Kirkham BW, Lassere MN, Edmonds JP, et al. Synovial membrane cytokine expression is predictive of joint damage progression in rheumatoid arthritis: A two-year prospective study (the DAMAGE study cohort) [J]. Arthritis Rheum, 2006, 54 (4): 1122-1131. [2]Rohn TA, J ennings GT, Hernandez M, et al. Vaccination against IL-17 suppresses autoimmune art hritis and encephalomyelitis [J]. Eur J Immunol, 2006, 36 (11):2857-2867. [3]Wong CK, Lit LC, Tam LS, et al. Hyperproduction of IL-23 and IL-17 in patients with systemic lupus erythematosus: implications for Th17-mediated inflammation in auto-immunity [J]. Clin Immunol, 2008, 127 (3): 385-393 [4] Murata M, Fujimoto M, Matsushita T, et al. Clinical association of serum interleukin-17 levels in systemic sclerosis: is systemic sclerosis a Th17 disease? [J]. J Dermatol Sci, 2008, 50 (3): 240-242. [5] Lowes MA, Kikuchi T, Fuentes-Duculan J, et al. Psoriasis vulgaris lesions contain discrete populations of Th1 and Th17 T Cells [J]. J Invest Dermatol, 2008, 128 (5): 1207-1211. [6] Koga C, Kabashima K, Shiraishi N, et al. Possible Pathogenic role of Th17 eells for atopic dermatitis [J].J Invest Dermatol, 2008, 128(11):2625-2630 [7] He D, Wu L, Kim H K, et al. CD8+IL-17-producing T cells are important in effector functions for the elicitation of contact hypersensitivity responses [J]. J Immunol, 2006, 177 (10): 6852-6858. [8] Cheung PF, Wong CK, Lam CW. Molecular mechanisms of cytokineand chemokine release from eosinophils activated by IL-17A, IL-17F, and IL-23: implication for Th17 lymphocytes-mediated allergic inflammation [J]. J Immunol, 2008; 180 (8): 5625 - 5635. [9] Aggarwal S ,Ghilardi N ,Xie MH et al .Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17 [J]. J Bio Chem, 2003; 278(3): 1910-1914. [10] Wolk K,Witte E ,Wallace E et al. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentation, and mobility in keratinocytes: a potential role in psoriasis [J]. Eur J Immunol, 2006; 36(5): 1309-1323. [11] Shen F, Gaffen SL. Structure-function relationships in the IL17 receptor: implications for signal transduction and therapy [J]. Cytokine, 2008; 41(2): 92-104. [12] Orihara K, Nakae S, Pawankar R, et al. Role of regulatory and proinflammatory T-cell populations in allergic diseases [J]. World Allergy Organization Journal, 2008, 1 (1): 9-14. [13] Ivanov II, McKenzie BS, Zhou L, et al. The orphan nuclear receptor RORγt directs the differentiation program of proinflammatory IL 17+Thelper cells [J]. Cell, 2006, 126(6): 1121-1133. [14] Basak PY, Adiloglu AK, Ceyhan AM，et al.The role of helper and regulatory T cells in the pathogenesis of vitiligo [J]. J Am Acad Detmatol, 2009; 60(2):256-260.