中波紫外线诱导小鼠皮肤光损伤中miRNA146a表达的研究

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (4): 267-270.

中波紫外线诱导小鼠皮肤光损伤中miRNA146a表达的研究

华丽娟¹,李巍¹,周炳荣²,骆丹³

1. 南京医科大学
2. 南京医科大学第一附属医院
3. 南京市南京医科大学附属第一医院皮肤科

收稿日期:2010-07-14 修回日期:2010-12-01 出版日期:2011-04-15 发布日期:2011-04-01
通讯作者: 骆丹 E-mail:daniluo2005@yahoo.com.cn
基金资助:
microRNA在光源性皮肤肿瘤发生中的作用及其机理研究

Expression of microRNA146a in Ultraviolet B （UVB）-damaged mouse skin

Received:2010-07-14 Revised:2010-12-01 Online:2011-04-15 Published:2011-04-01

摘要/Abstract

摘要：

目的探讨中波紫外线（UVB）诱导光损伤小鼠皮肤中miRNA146a表达的变化。方法以C57/BL6小鼠为研究对象，分时间组和剂量组，剂量组分别以30、60、90、180、270 mJ/cm2 UVB照射小鼠背部皮肤，24 h后取材；时间组以180 mJ/cm2 UVB照射小鼠背部皮肤后，取1、12、24、48 h为检测时点。采用实时荧光定量PCR检测miRNA146a的表达水平，同时利用在线数据库targetscan等预测靶基因，并用Gostat软件对其进行功能分析。免疫组化技术检测可能与其功能相关的信号转导通路的关键蛋白STAT3，初步探讨其功能。结果 UVB照射后，空白组，30、60、90、180、270 mJ/cm2剂量组miRNA146a的表达水平分别为0.01158 ± 0.00098、0.01083 ± 0.00104、0.00872 ± 0.00031、0.00851 ± 0.00033、0.00810 ± 0.00057、0.00770 ± 0.00031，与UVB的辐射剂量呈负相关（r = -0.83，P ＜ 0.05）；而在时间组中，UVB照射1、12、24、48 h后miRNA146a的表达水平分别为0.00730 ± 0.00036、0.00805 ± 0.00035、0.00810 ± 0.00057、0.00837 ± 0.00039，与对照组相比均有下调（P ＜ 0.05）。Gostat分析显示，miRNA146a主要与细胞生长分化以及多种信号转导途径相关。免疫组化结果提示，在高剂量UVB辐射时，磷酸化STAT3表达更为强烈。结论 miRNA146a可能在UVB诱导的光损伤机制中具有关键作用，主要体现与炎症负调控有关，可能通过JAK-STAT3信号转导途径起作用。

关键词: STAT3

Abstract:

Objective To investigate the expression changes of microRNA 146a （miRNA146a） in UVB-damaged mouse skin. Methods C57/BL6 mice were divided into dose groups to be irradiated on the back with UVB of 30, 60, 90, 180, 270 mJ/cm2 respectively for 24 hours, and time groups irradiated with UVB of 180 mJ/cm2 for 1, 12, 24 and 48 hours respectively. The mice received no irradiation served as the control. Skin samples were subsequently obtained from the irradiated sites of mice and subjected to real-time fluorescent PCR for the detection of miRNA146a expression as well as immunohistochemical staining （IHC） for the detection of STAT3. Results The expression levels of miRNA146a were 0.01158 ± 0.00098, 0.01083 ± 0.00104, 0.00872 ± 0.00031, 0.00851 ± 0.00033, 0.00810 ± 0.00057 and 0.00770 ± 0.00031 in the unirradiated control mice and mice irradiated with UVB of 30, 60, 90, 180, 270 mJ/cm2 for 24 hours, respectively, 0.00730 ± 0.00036, 0.00805 ± 0.00035, 0.00810 ± 0.00057 and 0.00837 ± 0.00039 in mice irradiated with UVB of 180 mJ/cm2 for 1, 12, 24 and 48 hours, respectively. IHC suggested an intensive expression of phosphorylated STAT3 in mice irradiated with a high dose UVB. Conclusions miRNA146a may play an essential role in the mechanism of UVB-induced photodamge, which is mainly correlated with the negative regulation of inflammatory reaction likely mediated by the JAK/STAT3 signal transduction pathway.

Key words: STAT3

华丽娟李巍周炳荣骆丹. 中波紫外线诱导小鼠皮肤光损伤中miRNA146a表达的研究[J]. 中华皮肤科杂志, 2011, 44(4): 267-270.

参考文献

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