TNF-α对HaCaT细胞核因子-κB/IκBα表达的调节作用

中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (11): 811-813.

TNF-α对HaCaT细胞核因子-κB/IκBα表达的调节作用

郁博¹,何威²,张斌³,杨玲⁴

1. 青岛大学医学院附属医院
2. 重庆市第三军医大学新桥医院皮肤科及风湿免疫科
3. 新桥医院皮肤科
4. 成都军区昆明总医院皮肤科

收稿日期:2010-03-02 修回日期:2010-07-01 出版日期:2010-11-15 发布日期:2010-11-10
通讯作者: 郁博 E-mail:yuboskin@126.com
基金资助:
第三军医大学第二附属医院1520人才基金资助课题

Modulatory effect of tumor necrosis factor α on the expression of nuclear factor-kappa Bα by a human keratinocyte cell line HaCaT

WEI HE²,zhang bin³, ³

Received:2010-03-02 Revised:2010-07-01 Online:2010-11-15 Published:2010-11-10

摘要/Abstract

摘要：

目的研究TNF-α对角质形成细胞NF-κB活性及IκBα表达的影响。方法采用反转录Real-Time PCR和Western blotting，观察经TNF-α作用下，培养的人角质形成细胞株HaCaT细胞的IκBα mRNA和蛋白质的表达情况，用Western blotting法检测NF-κB核蛋白的表达情况。结果 TNF-α可抑制HaCaT细胞IκBα的表达而诱导NF-κB的核转位，呈作用浓度依赖性效应，此作用在1h达到高峰，此后逐渐减弱。结论 TNF-α通过调控角质形成细胞NF-κB/IκB可参与皮肤的炎症反应。

关键词: 肿瘤坏死因子-α, 角质形成细胞, 核因子κB, IκB

Abstract:

Objective To investigate the effects of TNF-α on the regulation of NF-κB/IκB in human keratinocytes. Methods Cultured HaCaT cells were treated by TNF-α with different concentrations and for different durations. The expressions of IκBα mRNA and protein were examined by Reverse transcription-Real time polymerase chain reaction (RT-PCR) and Western blotting. The expressions of NF-κB neucleoprotein were examined by Western blotting. Results TNF-α had no effects on expression of GRα in HaCaT cells, but up-regulate the expression of GRβ in HaCaT cells in a time- and dose-dependent manner. Conclusion TNF-α might participates in inflammatory process via regulating NF-κB/IκB in keratinocytes.

Key words: TNF-α, keratinocytes, NF-κB, IκB

郁博何威张斌杨玲. TNF-α对HaCaT细胞核因子-κB/IκBα表达的调节作用[J]. 中华皮肤科杂志, 2010, 43(11): 811-813.

WEI HE zhang bin. Modulatory effect of tumor necrosis factor α on the expression of nuclear factor-kappa Bα by a human keratinocyte cell line HaCaT[J]. Chinese Journal of Dermatology, 2010, 43(11): 811-813.

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