中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (11): 766-769.

• 论著 • 上一篇    下一篇

尖锐湿疣组织中生存素、环氧合酶-2和血管内皮生长因子的表达及其与血管生成的关系

邓列华1,李璟蓉2,胡云峰1,许向前2,吴焱3,郭秀枝2,刘洁2,范洪涛2,林泽4,赵永铿2   

  1. 1. 广州市暨南大学附属第一医院皮肤科
    2.
    3. 北京地坛医院
    4. 广州暨南大学医学院附院皮肤科
  • 收稿日期:2010-03-08 修回日期:2010-04-06 出版日期:2010-11-15 发布日期:2010-11-10
  • 通讯作者: 邓列华 E-mail:liehuadeng@126.com
  • 基金资助:

    用抗HPV6L1细胞内抗体防治尖锐湿疣的研究

Expressions of survivin, COX-2, VEGF and their correlations with angiogenesis in condyloma acuminatum

  • Received:2010-03-08 Revised:2010-04-06 Online:2010-11-15 Published:2010-11-10
  • Contact: DENG Lie-hua E-mail:liehuadeng@126.com

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摘要：

目的 探讨生存素、环氧合酶-2（COX-2）、血管内皮生长因子（VEGF）在尖锐湿疣组织中的表达及其与尖锐湿疣血管生成的关系。方法 应用免疫组化PowerVision法对60例尖锐湿疣组织、21例正常包皮组织中生存素、COX-2、VEGF蛋白进行检测，根据CD105阳性的血管内皮细胞计数来测定尖锐湿疣组织微血管密度（MVD）。结果 生存素在尖锐湿疣组织中阳性表达率为56.67%，正常包皮组织中为9.52%；COX-2在尖锐湿疣组织中阳性表达率为63.33%，正常包皮组织中为0；两组比较，差异均有统计学意义（P值均 < 0.05）。生存素、COX-2在两组的阳性表达强度比较差异均有统计学意义（P值均 < 0.05）。VEGF在尖锐湿疣组织、正常包皮组织中阳性表达率均为100%，但两组阳性表达强度比较差异有统计学意义（P < 0.05）。尖锐湿疣组织的MVD值（16.38 ± 5.46）明显高于正常包皮组（0.62 ± 0.44），差异有统计学意义（P < 0.05）。在尖锐湿疣组织中，生存素、COX-2与VEGF蛋白表达两两之间，生存素与MVD，COX-2与MVD均呈显著正相关。结论 生存素、COX-2、VEGF在尖锐湿疣组织中表达程度均高于对照组。

关键词: 尖锐湿疣, Survivin, 环氧合酶-2, 血管内皮生长因子, CD105, 微血管密度, 免疫组化

Abstract:

Objective To investigate the expressions of survivin, cyclooxyenase-2 （COX-2） and vascular endothelial growth factor （VEGF） and their relationship with angiogenesis in condyloma acuminatum （CA） tissues. Methods Immunohistochemistry using PowerVision staining kit was performed to detect the expression of survivin, COX-2 and VEGF protein in 60 CA tissue samples from patients and 21 normal skin samples from the foreskin of human controls. At the same time, the microvessel density was determined in CA tissues by staining blood vessel endothelium with anti-CD105 monoclonal antibody. Results The positivity rate of survivin and COX-2 expression was 56.67% and 63.33%, in CA tissues, 9.52% and 0 in normal skin tissues, respectively. There was a significant difference between the two groups of tissue samples in the positivity rate and intensity of survivin and COX-2 expression（all P < 0.05）. VEGF was expressed in all of the CA tissues and normal skin tissues, while the intensity of VEGF expression was statistically different between the two groups of tissue samples（P < 0.05）. The MVD was 16.38 ± 5.46 and 0.62 ± 0.44 in CA tissues and normal skin tissues, respectively （P < 0.05）. There was a significant positive correlation between the expressions of survivin, COX-2 and VEGF, as well as between MVD and the expressions of survivin and COX-2 in CA tissues. Conclusion The expression levels of survivin, COX-2 and VEGF are significantly higher in CA tissues than in normal skin tissues.

Key words: Condyloma acuminatum, Survivin, COX-2, VEGF, CD105, MVD, IHC