低血清铁生殖道沙眼衣原体持续感染动物模型的研究

摘要/Abstract

摘要：

目的创建低血清铁生殖道沙眼衣原体持续感染的动物模型。方法用BALB/c雌性小鼠，通过缺铁饮食使其进入缺铁状态，小鼠阴道内注射衣原体标准株原体前1周肌内注射黄体酮，然后阴道内注射50 μl浓度约为3.4344 × 107 ifu/ml的E型沙眼衣原体标准株原体悬液。连续观察其阴道分泌物性状及免疫荧光镜检情况；并进行病理切片检测。进一步给予莫西沙星及铁剂干预，观察免疫荧光镜检情况。结果与正常小鼠比较，低血清铁小鼠生殖道沙眼衣原体感染时间明显延长，超过18周；18周后小鼠子宫，输卵管，卵巢病理切片呈现明显慢性炎症，淋巴细胞浸润。在后续给予小鼠莫西沙星及补铁干预后发现，单纯给予莫西沙星或补铁的小鼠免疫荧光重复多次检测并不阴转，而补铁后给予莫西沙星组阴转率达到60%。结论动物模型显示，低血清铁可能是生殖道沙眼衣原体持续感染的因素之一。

关键词: 沙眼衣原体, 持续感染, 动物模型

Abstract:

Objective To construct a model for persistent genital tract infection with Chlamydia trachomatis in mice with low serum iron. Methods Iron deficiency was induced in female BALB/c mice by low-iron diet. Mice with or without iron-deficency were inoculated intravaginally with direct instillation of 50 μl of bacterial suspension containing 3.4344 × 107 IFU/ml of C. trachomatis serovar E elementary body 1 week after intramuscular injection of progesterone. Moxifloxacin and ferrous sulfate, alone or in combination were given to inoculated mice with iron deficiency daily for 1 week. Subsequently, vaginal discharge was obtained from mice and observed under an immunofluorescence microscope, and tissue specimens were resected from the uterus，uterine tube and ovary of mice and subjected to pathological examination at various time points. Results Compared with normal-iron mice, iron-deficient mice were infected with C. trachomatis for a longer duration （more than 18 weeks）. After 18-week infection with C. trachomatis, there was an obvious chronic inflammation and lymphocytic infiltration in tissues specimens from the uterus，uterine tube and ovary of mice. Immunofluorescent examination of vaginal discharge for C. trachomatis turned negative in 60％ of iron-deficient mice treated with both moxifloxacin and ferrous sulfate, but not in any of those receiving moxifloxacin or ferrous sulfate alone. Conclusion As animal models show, low serum iron may contribute to the persistent genital tract infection with C. trachomatis.

Key words: Chlamydia trachomatis, Persistent infection, Animal model

刘勇刘原君姚卫锋盛彩虹朱辉李燕刘全忠. 低血清铁生殖道沙眼衣原体持续感染动物模型的研究[J]. 中华皮肤科杂志, 2010, 43(10): 702-704.

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