强脉冲光照射对小鼠皮肤胶原纤维、弹性纤维及透明质酸含量的影响

摘要/Abstract

摘要：

目的探讨强脉冲光照射对昆明小鼠皮肤胶原纤维、弹性纤维、透明质酸含量的意义。方法用强脉冲光照射小鼠右侧背部皮肤，左侧为对照，取各时间点照射组和对照组皮肤进行组织学观察，包括HE染色、Ⅰ型和Ⅲ型胶原纤维饱和苦味酸-天狼猩红染色、弹性纤维Gomori醛品红法染色，同时提取皮肤组织，碱水解-分光光度法测定羟脯氨酸含量，放射免疫法测定透明质酸含量。结果照射后2周，真皮厚度较对照组增加（t = 4.623，P < 0.05），4周时达最大值，较对照组增加18.71%，8周时仍大于对照组，差异有统计学意义（t = 3.904，P < 0.05）。照射后1周，Ⅲ型胶原纤维较对照组增加40.54%（t = 5.129，P < 0.05），照射后2周和4周，Ⅰ型和Ⅲ型胶原纤维均较对照组增多明显，至8周时，均大于对照组（P < 0.05）。照射后2 ~ 8周，弹性纤维均较对照组增多（P < 0.05）；照射后1 ~ 8周，羟脯氨酸含量均较对照组增多（P < 0.05）。照射后1 d和3 d，透明质酸含量显著增加，1 ~ 8周，渐进性降低，但均高于对照组（P < 0.05）。结论强脉冲光照射小鼠皮肤可刺激真皮胶原纤维、弹性纤维、透明质酸含量增加。

关键词: 强脉冲光, 胶原蛋白, 弹性纤维, 羟脯氨酸, 透明质酸

Abstract:

Objective To investigate the effects of intense pulsed light （IPL） irradiation on the content of collagen fibers, elastic fibers and hyaluronic acid in Kunming mouse skin. Methods The dorsal skin of mice was divided into two areas: the right area was irradiated with IPL, and the left remaining unirradiated served as the control. Skin specimens were taken from the back of mice on day 1, 3, week 1, 2, 4 and 8 after the irradiation and subjected to staining with HE, sirius red and Gomori aldehyde-fuchsin for examinations of histological changes, type I and Ⅲ collagen fibers and elastic fibers. The hydroxyproline and hyaluronic acid content in skin tissues of mice was determined with ultraviolet spectrophotometry and radioimmunoassay respectively. Results After irradiation, a significant increase was observed in dermal thickness on week 2 （t = 4.623, P < 0.05）, 4, and 8 （t = 3.904, P < 0.05）, in type Ⅲ collagen fiber （t = 5.129, P < 0.05） on week 1, in type I and Ⅲ collagen fibers on week 2, 4 and 8 （both P < 0.05）, in elastic fibers from week 2 to 8 （P < 0.05）, and in hydroxyproline content from week 1 to 8 （all P < 0.05） in the skin of mice compared with unirradiated mice. In detail, dermal thickness increased by 18.71% on week 4, and type Ⅲ collagen fiber by 40.54% in irradiated mice compared with unirradiated mice. Further more, the hyaluronic acid content was elevated from day 1 to 3, but gradually declined from week 1 to 8, and remained statistically higher from day 1 to week 8 （P < 0.05） in irradiated mice compared to unirradiated mice. Conclusion IPL irradiation could induce an increase in the content of collagen fiber, elastic fiber and hyaluronic acid in the dorsal skin of mice.

Key words: Intense pulsed light (IPL), Collagen, Elastic fibrin, Hydroxyproline, Hyaluronic acid

邓列华刘赛君胡云峰赵刚林泽赵永铿. 强脉冲光照射对小鼠皮肤胶原纤维、弹性纤维及透明质酸含量的影响[J]. 中华皮肤科杂志, 2010, 43(10): 705-708.

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