中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (10): 698-701.

• 论著 • 上一篇    下一篇

SLE患者外周血内皮祖细胞数量及生物学功能的研究

周培媚1,路永红1,熊霞2,程晓云3,朱颜俊3,曾世华3,陈涛4,瓦庆彪5,蔡琦6,陈前明7,蒋存火7   

  1. 1. 成都市第二人民医院皮肤科
    2. 四川省泸州医学院附院皮肤科
    3.
    4. 四川大学华西医院
    5. 成都市第二人民医院皮肤科；成都市皮肤性病防治研究所
    6. 北京大学人民医院皮肤科
    7. 成都市第二人民医院皮肤性病科
  • 收稿日期:2010-03-24 修回日期:2010-05-20 出版日期:2010-10-15 发布日期:2010-10-09
  • 通讯作者: 熊霞 E-mail:xiongxia789@126.com
  • 基金资助:

    四川省卫生厅科研课题

Changes in the number and biological ability of endothelial progenitor cells from peripheral blood of patients with systemic lupus erythematosus （SLE

  • Received:2010-03-24 Revised:2010-05-20 Online:2010-10-15 Published:2010-10-09

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摘要：

目的 探讨SLE患者外周血内皮祖细胞数量和功能的变化。方法 女性SLE患者及正常人对照各20例，取外周血20ml，密度梯度离心法分离单一核细胞，采用CD133/CD34、CD133/VEGFR2双荧光标记鉴定细胞，CD34/CD133/VEGFR2三荧光标记流式检测EPC细胞数量，采用MTT比色法、改良的millicell小室和黏附能力测定实验观察EPC的增殖、迁移和黏附能力。结果 SLE患者外周血内皮祖细胞数量较正常人对照明显下降（4.49% ± 1.66%比20.81% ± 4.14%，P < 0.01），其增殖（23.11 ± 3.16比35.65 ± 1.74）、迁移（12.00 ± 2.12个细胞/视野比23.60 ± 3.0个细胞/视野）、黏附能力（22.43 ± 4.43个细胞/视野36.43 ± 3.69个细胞/视野）均有所下降（P < 0.01）。结论 SLE患者外周血内皮祖细胞数量减少、功能受损。

关键词: 系统性红斑狼疮, 内皮祖细胞

Abstract:

Objective To analyze the changes in number and biological ability of endothelial progenitor cells （EPCs） from peripheral blood of SLE patients. Methods Mononuclear cells （MNCs） were isolated by Ficoll density gradient centrifugation from peripheral blood of 20 female SLE patients and 20 healthy female controls. EPCs were identified by double staining using antibodies to CD34 and CD133, or antibodies to CD133 and vascular endothelial growth factor receptor 2 （VEGFR2）. Phycoerythrin （PE） conjugated anti-CD34, fluorescein isothiocyanate （FITC） conjugated anti-CD133 and APC conjugated anti-VEGFR2 antibodies were used in a three color flow cytometric analysis to determine the percentage of EPCs in peripheral MNCs. The proliferation and migration ability of EPCs were measured by MTT assay and modified millicell chamber assay, respectively. The adhesion activity of EPCs was evaluated by counting the number of adherent cells. Results The percentage and proliferation rate of EPCs in peripheral MNCs from female SLE patients were significantly lower than those from the healthy controls（4.49% ± 1.66% vs 20.81% ± 4.14%, 23.11% ± 3.16% vs 35.65% ± 1.74%, both P < 0.01）. The migration and adhesion ability of EPCs from SLE patients was impaired compared with those from the healthy controls （12.00 ± 2.12 vs 23.60 ± 3.0 cells/field, 22.43 ± 4.43 vs 36.43 ± 3.69 cells/filed, both P < 0.01）. Conclusion There is a decrease in the number and an impairment in biological ability of EPCs in SLE patients.

Key words: Systemic lupus erythematosus, Endothelial progenitors cells