中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (9): 647-647.

• 短篇论著 • 上一篇    下一篇

巨噬细胞是正常成人真皮中主要的细胞成分

郭英军1,赵玉铭2,王雅坤2,陈洪铎3   

  1. 1. 沈阳市中国医科大学附属盛京医院皮肤科
    2. 沈阳 中国医科大学附属第一医院皮肤科
    3. 中国医科大学第一附属医院皮肤科
  • 收稿日期:2008-07-14 修回日期:2008-09-24 出版日期:2009-09-15 发布日期:2012-03-21
  • 通讯作者: 郭英军 E-mail:yingjunguo@126.com
  • 基金资助:
    沈阳市科委资助课题（1071200-1-00）

Macrophages constitute a major subpopulation of cells in the human dermis

2,CHEN Hong-Duo 2   

  • Received:2008-07-14 Revised:2008-09-24 Online:2009-09-15 Published:2012-03-21

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摘要： 目的 研究正常人真皮内的巨噬细胞和树枝状细胞占所有有核细胞的比率。方法 正常人8例，每例均取面部、躯干、四肢近端、四肢远端、手掌和足跖6个部位皮肤，进行铺片和纵行与水平连续切片。CD68,CD36单克隆抗体和FXIIIa多克隆抗体染色。观察这些巨噬细胞胞占真皮中所有有核细胞的比例。结果 CD68阳性巨噬细胞占真皮浅层所有有核细胞总数的67.5±17.1%， FXIIIa阳性真皮树枝状细胞为35.2±14.5%，CD36阳性细胞为27.0±11.4%。结论 巨噬细胞不仅是正常成人真皮中的主要的免疫细胞，而且是真皮内皮肤免疫防御功能的主要细胞。在真皮内还有一定数量的抗原提呈细胞。

关键词: 真皮, 巨噬细胞

Abstract: Macrophages constitute a major subpopulation of cells in the human dermis. Yingjun GUO, Yuming ZHAO, Yakun WANG, Hong-Duo CHEN, Department of Dermatology, the first Hospital of China Medical University, Shenyang 110001,China [Abstract] Objective To observe the percent of macrophages and dendritic cells in normal adult human dermis. Methods Normal skin from 6 locations including the face, trunk, proximal limbs, distal limbs, and palms and soles of 8 subjects were included in the study. The epidermal sheets, horizontal and longitudinal sections of the skin were stained with an ABC immunoperoxidase procedure with anti–CD68，CD36 monoclonal and anti-FXIIIa polyclonal antibodies. Results The percent of CD68 positive cells were 67.5±17.1% within the superficial dermis, FXIIIa were 35.2±14.5%, CD36 were 27.0±11.4%.Conclusion: Macrophages are the main cells within the normal human dermis with immune defense functions. There are also significant numbers of dermal dendritic cells within the normal dermis.