中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (8): 556-559.

• 论著 • 上一篇    下一篇

药疹患者Epstein-Barr病毒感染的检测

朱桂芝1,陈官芝2,罗兵3   

  1. 1. 青岛市中心医院皮肤科
    2. 青岛大学医学院附属医院皮肤科
    3. 青岛大学医学院微生物教研室
  • 收稿日期:2008-07-21 修回日期:2008-09-01 出版日期:2009-08-15 发布日期:2009-08-10
  • 通讯作者: 朱桂芝

Preliminary study on Epstein-Barr virus infection in patients with drug eruption

  • Received:2008-07-21 Revised:2008-09-01 Online:2009-08-15 Published:2009-08-10

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摘要：

目的 探讨Epstein-Barr病毒（EBV）感染在药疹发病中的作用。方法 应用PCR-Southern杂交技术检测32例药疹患者和30例正常人对照外周血单一核细胞中EBV特异性DNA片段BamHⅠ-W，筛选出EBV阳性标本，对EBV阳性标本进行RT-PCR和Southern杂交，检测EBV即刻早期基因BZLF1的表达；同时采用ELISA检测血清EBV衣壳抗原（VCA）-IgM抗体。结果 药疹患者和正常人对照EBV DNA阳性率分别为78.13%（25/32）和10.00％（3/30），药疹患者组明显高于正常人对照组（P < 0.01）；轻型药疹和重型药疹患者阳性率的差异无统计学意义（P = 0.104）。25例药疹患者EBV DNA阳性标本中有3例EBV BZLF1 mRNA表达阳性，其中轻型药疹患者1例，重型药疹患者2例。32例药疹患者组中有6例EBV VCA-IgM抗体阳性，轻型和重型药疹患者阳性率差异无统计学意义（P = 0.672）；30例对照组均为阴性。结论 药疹患者中存在EBV的活动性感染，EBV感染可能是影响药疹发病的环境因素之一。

关键词: 药疹;爱泼斯坦巴尔病毒感染;基因，即早;免疫球蛋白M

Abstract:

Objective To explore the role of Epstein-Barr virus （EBV） infection in the etiology of drug eruption. Methods PCR-Southern blot was used to detect EBV-specific DNA fragment BamHⅠ-W in peripheral blood mononuclear cells of 32 patients with drug eruption and 30 age- and sex-matched normal controls. The mRNA expression of EBV lytic gene BZLF1 in EBV DNA-positive samples was measured by RT-PCR and Southern blot. ELISA was performed to detect EBV virus capsule antigen （VCA）-specific IgM. Results The positivity rate of EBV DNA was significantly higher in patients with drug eruption than in normal controls （78.13％ （25/32） vs 10.00％ （3/30）, P < 0.01）, while no significant difference was noted between patients with severe and mild drug eruption （P > 0.05）. The expression of BZLF1 mRNA was detected in 3 out of 25 EBV DNA-positive patients; of the 3 patients, 1 suffered from mild drug eruption, and 2 from severe drug eruption. EBV VCA-specific IgM was observed in 6 of 32 patients with drug eruption, but not in any normal controls. No significant difference in the positivity rate of EBV VCA-specific IgM existed between patients with severe and mild drug eruption （P > 0.05）. Conclusions There is an active infection of EBV in patients with drug eruption. EBV infection is probably an environmental factor affecting the development of drug eruption.

Key words: Drug eruptions; Epstein-Barr virus infection;Genes,immediate-early; Immunoglobulin M