中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (8): 553-555.

• 论著 • 上一篇    下一篇

慢性荨麻疹患者血浆RANTES、嗜酸粒细胞趋化因子、TNF-α与白三烯B4水平的测定

史立宏1,李晓民2,李金勇2,牟华光2   

  1. 1. 潍坊医学院
    2. 潍坊市皮肤病医院
  • 收稿日期:2008-06-04 修回日期:2009-03-06 出版日期:2009-08-15 发布日期:2009-08-10
  • 通讯作者: 史立宏

Detection of plasma RANTES, eotaxin, TNF-α and LTB4 in patients with chronic urticaria

  • Received:2008-06-04 Revised:2009-03-06 Online:2009-08-15 Published:2009-08-10

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摘要：

目的 探讨慢性荨麻疹患者血浆中调节激活正常T细胞表达和分泌的细胞因子（RANTES）、嗜酸粒细胞趋化因子（eotaxin）、TNF-α及白三烯B4（LTB4）的水平及其意义。方法 对41例慢性荨麻疹患者进行临床评价，按症状积分将病情分为轻型、中型、重型3级。应用咪唑斯汀10 mg每日1次，连续治疗4周。采用ELISA法测定20例健康志愿者与41例患者治疗前后血浆中RANTES、eotaxin、TNF-α与LTB4的水平。结果 ①慢性荨麻疹组血浆中RANTES、eotaxin、TNF-α和LTB4水平分别为（52.5 ± 10.2）μg/L、（58.4 ± 16.1） μg/L、（35.1 ± 9.6） ng/L和（109.4 ± 21.7） ng/L，健康对照组水平分别为（33.7 ± 9.4） μg/L、（48.3 ± 13.6） μg/L、（21.3 ± 8.9） ng/L和（77.8 ± 11.6） ng/L，两组比较差异均有统计学意义，P值分别 < 0.01、 < 0.05、 < 0.01、 < 0.01。②血浆RANTES、eotaxin、TNF-α、LTB4水平与临床表现的相关性分析显示：中、重型慢性荨麻疹患者的RANTES、eotaxin、TNF-α与LTB4血浆水平高于轻型患者，差别均有统计学意义，P < 0.05；重型患者的RANTES、eotaxin、TNF-α与LTB4血浆水平较中型患者略有增高，但差异均无统计学意义，P > 0.05。③咪唑斯汀治疗后慢性荨麻疹组血浆中RANTES、eotaxin、TNF-α和LTB4水平较前明显下降，分别为（36.3 ± 8.9） μg/L、（46.3 ± 10.2） μg/L、（23.2 ± 7.5） ng/L和（83.1 ± 14.2） ng/L，与治疗前比较差异有统计学意义，P值均 < 0.01，与健康对照组比较P值均 > 0.05。结论 慢性荨麻疹患者血浆RANTES、eotaxin、TNF-α与LTB4水平升高，并与病情的严重程度呈正相关趋势，咪唑斯汀治疗后血浆中RANTES、eotaxin、TNF-α和LTB4水平较前明显下降，提示RANTES、eotaxin、TNF-α与LTB4在慢性荨麻疹的发病过程中可能起一定作用。

Abstract:

Objective To investigate the plasma levels of regulated upon activation normal T cell expressed and secreted （RANTES）, eotaxin, tumor necrosis factor （TNF）-α and leukotriene B4 （LTB4） in patients with chronic urticaria and their roles in the pathogenesis of chronic urticaria. Methods Forty-one patients with chronic urticaria were included into this study along with 20 normal human controls. Patients were graded into three groups, i.e. mild group （n = 11）, moderate group （n = 21） and severe group （n = 9）, according to their symptom score. All patients were treated with mizolastine 10 mg per day for 4 weeks. ELISA was used to study the plasma levels of RANTES, eotaxin, TNF-α and LTB4 in normal controls and patients before and after treatment. Results The plasma levels of RANTES, eotaxin, TNF-α and LTB4 were （52.5 ± 10.2） g/L, （58.4 ± 16.1） g/L, （35.1 ± 9.6） ng/L and （109.4 ± 21.7） ng/L, respectively, in untreated patients with chronic urticaria, compared to （33.7 ± 9.4） g/L, （48.3 ± 13.6） g/L, （21.3 ± 8.9） ng/L and （77.8 ± 11.6） ng/L, respectively, in normal controls（P < 0.01, 0.05, 0.01, 0.01, respectively）. Increased plasma levels of RANTES, eotaxin, TNF-α and LTB4 were observed in patients with moderate or severe chronic urticaria compared with those with mild chronic urticaria （both P < 0.05）, whereas there was no significant difference between patients with severe and mild chronic urticaria （P < 0.05）. After treatment with mizolastine the plasma levels of RANTES, eotaxin, TNF-α and LTB4 were （36.3 ± 8.9） g/L, （46.3 ± 10.2） g/L, （23.2 ± 7.5） ng/L and （83.1 ± 14.2） ng/L respectively, significantly lower than those in patients before treatment （all P < 0.01）, but showed no difference from those in normal controls （all P > 0.05）. Conclusions The plasma levels of RANTES, eotaxin, TNF-α and LTB4 are elevated in patients with chronic urticaria, and they exhibits a positive correlation tendency with disease activity. After treatment with mizolastine, a significant decrease is observed in the plasma levels of RANTES, eotaxin, TNF-α and LTB4, which hints that RANTES, eotaxin, TNF-α and LTB4 may play a certain role in the pathogenesis of chronic urticaria.