中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (4): 264-266.

• 短篇论著 • 上一篇    下一篇

蕈样肉芽肿中P16INK4a基因甲基化状态及其蛋白的表达

崔成军1,谢维佳2,施启丰2,张新华1,吴波1   

  1. 1. 南京军区总医院病理科
    2.  无锡锡山人民医院
  • 收稿日期:2008-03-12 修回日期:2008-04-13 出版日期:2009-04-15 发布日期:2009-04-14
  • 通讯作者: 崔成军
  • 基金资助:

    江苏大学临床医学基金资助 项目编号JLY20050071;不符合上述请自行填写具体基金资助项目名称（编号）

DNA methylation and expression of P16 (INK4a) in tissues of mycosis fungoides

  • Received:2008-03-12 Revised:2008-04-13 Online:2009-04-15 Published:2009-04-14

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摘要：

[摘要] 目的：探讨蕈样肉芽肿和大斑片样副银屑病中P16INK4a基因甲基化状态和其蛋白的表达对其分型的相关性影响。方法：采用免疫组织化学（SP法）以及甲基化特异性PCR（MSP）法检测14例蕈样肉芽肿和4例大斑片样副银屑病石蜡蜡块组织中P16INK4a蛋白及P16INK4a启动子区甲基化状态。结果：蕈样肉芽肿中14例中有9例少量表达P16INK4a蛋白，10例P16INK4a甲基化阳性，大斑片样副银屑病4例中有1例少量表达P16INK4a蛋白，4例全部P16INK4a甲基化阳性。蕈样肉芽肿组和大斑片样副银屑病组之间以及蕈样肉芽肿各组之间的蛋白表达和甲基化状态无统计学差异（P>0.05）。结论：P16INK4a基因甲基化状态及蛋白的表达和蕈样肉芽肿的分型可能不具有相关性。

Abstract:

[Abstract] Objective To investigate aberrant methylation in the promoter area of P16INK4a gene and its protein expression in mycosis fungoides and large plaque parapsoriasis, and to evaluate the association with the different type of mycosis fungoides. Methods Immunohistochemistry and methylation-specific PCR(MSP) were performed on 14 cases of mycosis fungoides and four cases of large plaque parapsoriasis in paraffin embeded samples to detect the expression level of P16INK4aprotein and its methylation state. Results In mycosis fungoides group, nine cases expressed low level of P16INK4a protein and ten cases diplayed P16INK4a methylation. In large plaque parapsoriasis group, only one case expressed low level of P16INK4a protein, all four cases showed P16INK4a methylation. There was no significantly difference between the mycosis fungoides group and the large plaque parapsoriasis group, and there was no significantly difference among different stages of mycosis fungoides（P>0.05）.Conclusion The methylation status of P16INK4a and the expression of its protein may have no relationship with the different type of mycosis fungoides.