中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (11): 748-750.

• 论著 • 上一篇    下一篇

人疱疹病毒8型ORF75基因亚型与Kaposi肉瘤的相关性研究

马春雷1,普雄明2,吴卫东3,张德志4,吴秀娟5   

  1. 1. 新疆维吾尔自治区人民医院皮肤性病科（现在新疆伊犁州友谊医院皮肤科，地址：新疆伊宁市斯大林街92号，835000）
    2. 乌鲁木齐市新疆维吾尔自治区人民医院皮肤科
    3. 新疆维吾尔自治区人民医院
    4. 乌鲁木齐新疆维吾尔自治区人民医院皮肤科
    5. 新疆维吾尔自治区人民医院皮肤性病科
  • 收稿日期:2008-11-21 修回日期:2008-12-14 出版日期:2009-11-15 发布日期:2009-11-13
  • 通讯作者: 马春雷 E-mail:mcl522@sohu.com

Study on the ORF75 subtypes of human herpesvirus–8 in Xinjiang patients with Kaposi’s sarcoma

  • Received:2008-11-21 Revised:2008-12-14 Online:2009-11-15 Published:2009-11-13
  • Contact: 马春雷 MA Chun-Lei E-mail:mcl522@sohu.com

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摘要：

目的 明确新疆Kaposi 肉瘤患者感染人类疱疹病毒8型（HHV-8）ORF75基因亚型分类,初步探讨其与新疆KS不同临床分型及侵袭性的相关性。方法 对25例新疆Kaposi 肉瘤石蜡包埋组织进行HHV-8 DNA抽提、扩增及双向测序，使用Clustal W软件和PHYLIP软件包对测序结果进行系统发生学分析，从而确定HHV-8 ORF75基因亚型，最后运用Fisher确切概率法对结果进行统计学分析。结果 ①25例新疆Kaposi 肉瘤有21例HHV-8感染为阳性，阳性率为84%，其中7例AIDS-KS患者HHV-8感染均为阳性。②21例HHV-8 阳性患者中，18例为HHV-8 ORF75 A亚型，3例为C亚型；③不同亚型间Kaposi肉瘤患者有无黏膜损害及临床分型的分布差异均无统计学意义（P>0.05）。结论 ① ORF75基因可以作为HHV-8流行病学研究的主要检测基因；②新疆KS患者感染HHV-8 ORF75亚型属于A亚型和C亚型；③HHV-8 ORF75不同亚型与新疆KS黏膜损害及临床分型无关。

关键词: 肉瘤，卡波西, 疱疹病毒8型,人, 开放读码柜架, 基因型

Abstract:

Objective: To study the epidemic situation and characteristic of ORF75 subtypes of Human herpesvirus-8 in Kaposi′ s Sarcoma in Xinjiang, and to primary discuss the correlations between different forms of Kaposi’s sarcoma and different subtypes. Methods: HHV-8 DNA were amplified by nested-PCR and sequenced which was extracteded from 25 cases paraffin-embedded tissue from the Xinjiang .The phylogenetic analysis were carried out by using the software DNASTAR, the program Clustal W and the PHYLIP package and then the ORF75 subtype was confirmed . Correlations between different clinics of Kaposi’s sarcoma and different subtypes were determined by Fisher’s exact probabilities. Results:　①HHV-8 DNA was detected in 25(84%) out of 21 cases and 7 cases AIDS-KS (100%) in Xinjiang . ②Based on the partial ORF75 nucleotide sequence analysis, indicate that Eighteen of these strains belong to the A subtype , with three belonging to the C group.③The different ORF75 subtypes of HHV-8 among mucosa damage and clinical classification of Kaposi′ s Sarcoma were not significant (P >0.05).Conclusion:①ORF75 gene is detected as epidemiologic study of HHV-8. ②The majority of Kaposi′ s Sarcoma patients in Xinjiang were infected by the A and C subtype of HHV-8. ③The different ORF75 subtypes of HHV-8 have nothing to do with mucosa damage and clinical classification of Kaposi′ s Sarcoma.

Key words: Human herpesvirus 8