中华皮肤科杂志

• 论著 • 上一篇    下一篇

血管内皮生长因子受体家族在HaCaT细胞中的表达

杨晓红, 满孝勇, 卜璋于, 蔡绥勍, 郑敏   

  1. 浙江大学医学院附属第二医院皮肤科, 杭州310009
  • 收稿日期:2006-07-04 出版日期:2007-05-15 发布日期:2007-05-15
  • 通讯作者: 郑敏,email:minz@zju.edu.cn E-mail:minz@zju.edu.cn
  • 基金资助:
    周家自然科学基金(30471565)

Expression of vascular endothelial growth factor receptors in HaCaT cells

YANG Xiao-hong, MAN Xiao-yong, BU Zhang-yu, CAI Sui-qing, ZHENG Min   

  1. 浙江大学医学院附属第二医院皮肤科, 杭州310009
  • Received:2006-07-04 Online:2007-05-15 Published:2007-05-15

PDF (PC)

76

赞

摘要： 目的 探讨血管内皮生长因子受体(VEGFR)家族在角质形成细胞系HaCaT细胞的表达。方法 RT-PCR法检测HaCaT细胞中VEGFR家族中VEGFR-1、VEGFR-2、VEGFR-3及neuropilin (NRP)-1、NRP-2的mRNA表达,蛋白免疫印迹法检测VEGFR家族蛋白质的表达,同时免疫荧光法定位VEGFR家族在HaCaT细胞中的表达情况。结果 RT-PCR发现VEGFR-1、VEGFR-2、VEGFR-3及NRP-1、NRP-2 mRNA在HaCaT细胞中均有表达;蛋白免疫印迹发现VEGFR-1、VEGFR-2、VEGFR-3蛋白质相对分子质量均为180 000,NRP-1、NRP-2为140 000。HaCaT细胞膜及细胞质内检测到较强的VEGFR家族荧光信号,以细胞膜表达为强。结论 在mRNA及蛋白质水平,HaCaT细胞均表达VEGFR-1、VEGFR-2、VEGFR-3及NRP-1、NRP-2。

关键词: 角蛋白细胞, 受体, 血管内皮生长因子, HaCaT细胞

Abstract: Objective To measure the expression of vascular endothelial growth factor receptors (VEGFR)in a keratinocyte cell line of HaCaT.Methods RT-PCR was used to measure the mRNA expression level of VEGFR,including VEGFR-1,-2,-3,neuropilin-1(NRP-1)and NRP-2 in HaCaT cells.Western blot was used to determine the protein expression of VEGFR,and immunofluorescence to locate the expression of VEGFR in these cells.Results Both the mRNA and protein expressions of VEGFR-1,-2,-3, NRP-1 and -2 were detected in HaCaT cells.The molecular weights of VEGFR-1,-2,-3 were shown to be 180000,and those of NRP-1 and -2 to be 140 000 by Western blot.Fluorescence signals for VEGFR were observed in both the membrane and cytoplasm of HaCaT cells,and the signals were stronger in the mem-brane than in the cytoplasm.Conclusion VEGFR-1,-2,-3,NRP-1 and -2 are all expressed in HaCaT cells at mRNA and protein levels.

Key words: Keratinocytes, Receptors,vascular endothelial growth factor, HaCaT cell