中华皮肤科杂志

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中波紫外线通过EGFR/P13K/AKT途径上调HaCaT细胞转铁蛋白受体的表达

李燕华, 夏济平, 王小勇, 康健, 孙蔚凌, 王飞, 相文忠, 蒋艺, 毕志刚   

  1. 南京医科大学第一附属医院皮肤科, 210029
  • 收稿日期:2006-02-27 出版日期:2007-10-15 发布日期:2007-10-15
  • 通讯作者: 毕志刚,email:eltonbi@21cn.com E-mail:eltonbi@21cn.com
  • 基金资助:
    国家自然科学基金(30271195，30671894);江苏省重点学科基金(135-03)

Ultraviolet B irradiation upregulates the expression of transferrin receptor via the EGFR/PI3K/AKT signaling pathway in HaCaT cells

LI Yan-hua, XIA Ji-ping, WANG Xiao-yong, KANG Jian, SUN Wei-ling, WANG Fei, XIANG Wen-zhong, JIANG Yi, BI Zhi-gang   

  1. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2006-02-27 Online:2007-10-15 Published:2007-10-15

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摘要： 目的 探讨中波紫外线辐射对HaCaT转铁蛋白受体表达的影响及其信号途径。方法 流式细胞仪检测HaCaT细胞转铁蛋白受体的表达。实时PCR检测转铁蛋白受体mRNA表达。结果 UVB辐射可上调HaCaT细胞转铁蛋白受体mRNA及其蛋白的表达,并在一定范围内(10 mJ/cm2～30 mJ/cm2)呈剂量依赖性。表皮生长因子受体(EGFR)抑制因子PD153035、磷脂酰肌醇3激酶(PI3K)抑制因子LY294002和渥曼青霉素(wortmannin)均可显著抑制UVB辐射对转铁蛋白受体的上调作用(P<0.01)。结论 紫外线可通过EGFR/PI3K/AKT途径上调HaCaT细胞转铁蛋白受体的表达。

关键词: 紫外线, 受体, 表皮生长因子, 1-磷脂酰肌醇3-激酶, 受体, 转铁蛋白

Abstract: Objective To investigate the effect of ultraviolet B(UVB)irradiation on the expression of transferrin receptor(TfR),and the underlying mechanism for such an expression,in HaCaT cells. Methods Cultured HaCaT cells were irradiated with different doses ofUVB(0,10,20,30 mJ/cm2),followed by another 24-hour culture.The epidermal growth factor receptor inhibitor PD153035,PI3K inhibitor LY294002,and wortmannin,respectively,were used to treat the cultured HaCaT cells before the irradiation. The expression of TfR mRNA was assessed by real-time reverse transcription PCR at 0,2,4,8,16 h after UVB irradiation.The expression of TfR protein was detected by flow cytometry using anti-human TfR-FITC monoclonal antibody at 0,6,12,and 24 h after the irradiation.Results Within a dose range of 10-30 mJ/cm2, ultraviolet B irradiation upregulated the mRNA and protein expressions of TfR in a dose-dependent manner. This upregulation was significantly inhibited by PD153035,LY294002,or wortmannin(all P<0.01).Conclusion These results suggest that ultraviolet B irradiation upregulates the expression of TfR via the EGFR/PI3K/AKT signaling pathway in HaCaT cells.

Key words: Ultraviolet rays, Receptor, epidermal growth factor, 1-Phosphatidylinositol 3-kinase, Receptors, transferrin