中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (1): 13-15.

• 论著 • 上一篇    下一篇

自体外周血干细胞移植治疗难治性天疱疮一例

曾抗1, 孙竞2, 孙乐栋1, 孟凡义2, 刁友涛1, 徐丹2, 黄良1, 赵洁2, 刘启发2   

  1. 1. 南方医科大学南方医院皮肤科 广州 510515;
    2. 南方医科大学南方医院血液科
  • 收稿日期:2006-02-11 出版日期:2007-01-15 发布日期:2007-01-15
  • 通讯作者: 孙乐栋,email:sunledong126@126.com E-mail:sunledong126@126.com

Autologous peripheral hematopoietic stem cell transplantation in a patient with refractory pemphigus

ZENG Kang1, SUN Jing2, SUN Le-dong1, MENG Fan-yi2, DIAO You-tao1, XU Dan2, HUANG Liang1, ZHAO Jie2, LIU Qi-fa2   

  1. Departrnent of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
  • Received:2006-02-11 Online:2007-01-15 Published:2007-01-15

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摘要： 目的 探讨自体外周血干细胞移植治疗天疱疮的可能性.病历资料男性患者,35岁,全身反复大疱4年余,根据临床表现、组织病理和免疫荧光检查确诊为天疱疮.经糖皮质激素和免疫抑制剂治疗效果不佳,2月前出现髓关节疼痛,经X线和CT检查诊断为股骨头无菌性坏死.采取环磷酞胺及粒细胞集落刺激因子作为动员剂,利妥昔单抗联合净化.用环磷酞胺联合抗胸腺细胞球蛋白、利妥昔单抗进行预处理,同时碱化和水化尿液,保护心、肝和肾功能.观察移植后临床表现、造血和免疫指标等的变化.结果 患者移植结束即停用糖皮质激素,3周后自觉双侧靛关节疼痛减轻,复查CT示股骨头缺血性改变减轻.移植6周后直接免疫荧光和间接免疫荧光检查转阴并持续阴性.经一年复诊,临床及免疫学检查均无复发迹象.结论 本研究结果提示自体外周血干细胞移植治疗天疙疮近期疗效显著,可能是治疗存在治疗抵抗的重症天疤疮潜在的“根治”方法,但其风险收益比尚需进一步评价.

关键词: 天疱疮, 造血干细胞, 移植,自体, 利妥昔单抗

Abstract: Objective To explore the possibility of autologous peripheral hematopoietic stem cell transplantation in treatment with refractory pemphigus. Patient and Intervention A 35-year-old male patient presented with a 4-year history of recurrent bullae on his trunk and extremities. The diagnosis of pemphigus was made on the basis of the clinical, histologic, and immunofiuorescence findings. The patient showed resistance to conventional therapy with glucocorticoid and immunosuppressive agents. Two months before admission, he complained of hip joint pain, and X-ray and CT scan revealed amicrobic necrosis of the femoral head. Stem-cell mobilization was achieved by treatment with cyclophosphamide, granulocyte colony-stimulating factor(G-CSF),and rituximab. Peripheral blood stem cells were collected via leukapheresis and cryopreserved for later use. Immunoablation was accomplished using cyclophosphamide(200mg/kg; divided 50 mg/kg on days -5,-4,-3, and -2),antithyrnocyte globulin(ATG; 10 mg/kg; divided 2.5mg/kg on days -6,-5,-4, and -3),and rituximab(1200 mg/d; divided 600mg/d on days 0 and 7).Autologous peripheral hematopoietic stem cell transplantation was followed by reconstitution of the immune system, which was monitored by flow cytometry. Results The glucocorticoid was withdrawn immediately after transplantation. His pemphigus titerturned negative at 6 weeks posttransplantation and remained negative. The patient was in complete drug-free.remission with no evidence for residual clinical or serological activity of pemphigus during 1 year of follow-up. Conclusions The patient's response suggests that autologous peripheral hematopoietic stem cell transplantation may be a potential "cure" for refractory pemphigus.Therefore, this approach deserves further studies to evaluate the risk-benefit ratio in patients with pemphigus showing resistance to conventional therapy.

Key words: Pemphigus, Hematopoietic stem cells, Transplantation, autologous, Rituximab