CD147 siRNA对恶性黑素瘤细胞株A375增殖及CD147表达的影响

中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (4): 204-206.

CD147 siRNA对恶性黑素瘤细胞株A375增殖及CD147表达的影响

陈翔, 林静, 林伟, 粟娟, 陈明亮, 施为, 谢红付, 陈服文

中南大学湘雅医院皮肤科长沙 410008

收稿日期:2005-08-24 发布日期:2006-04-15
基金资助:
国家自然科学基金(30200248);教育部高等院校青年教师基金(2003-189);霍英东高校青年教师基金资助项目(91040)

Effects of siRNA targeting CD147 gene on the expression of CD147 and the proliferation of malig- nant melanoma cell line A375

CHEN Xiang, LIN Jing, LIN Wei, SU Juan, CHEN Ming-liang, SHI Wei, XIE Hong-fu, CHEN Fu-wen

Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China

Received:2005-08-24 Published:2006-04-15

摘要/Abstract

摘要： 目的研究CD147siRNA转染对恶性黑素瘤细胞株A375中CD147表达及细胞增殖的影响。方法将前期构建的重组质粒pSUPER/CD147 siRNA稳定转染至恶性黑素瘤细胞株A375中,采用半定量RT-PCR法检测转染细胞中CD147 mRNA的表达。采用MTT法检测转染pSUPER/CD147 siRNA对肿瘤细胞增殖的影响。结果转染了pSUPER/CD147 siRNA的恶性黑素瘤细胞株A375中CD147 mRNA表达水平显著下调,其在转染后24h、48h和72h的增殖水平均显著下降。结论 siRNA转染能有效抑制恶性黑素瘤细胞株A375中CD147的表达,且能抑制肿瘤细胞的增殖能力。

关键词: RNA,小分子干扰, 黑色素瘤, 细胞增殖, CD147

Abstract: Objective To investigate the effects of siRNA targeting CD 147 gene on the expression of CD147 in melanoma cell line A375, and on proliferation of these cells. Methods Previously prepared recombinant plasmid pSUPER/CD147 siRNA was used. In this study, the recombinant was transfected into the A375 cells. The mRNA expression of CD147 was measured by semi-quantitive RT-PCR, the proliferation of the cells by MTT assay. Results After the transfection with pSUPER/CD147 siRNA1 and pSU-PER/CD147 siRNA2, the mRNA expression of CD147 in the A375 cells was significantly down-regulated by 90.81% (P<0.001) and 13.37% (P<0.05) respectively. The proliferation levels of transfected tumor cells at 24, 48 and 72 h after the transfection with pSUPER/CD147 siRNA1 also decreased significantly by 49.42%, 52.60% and 53.33% respectively (P<0.05,<0.001,<0.001, respectively). Conclusion siRNA targeting CD147 gene could effectively inhibit the expression of CD147 in A375 cells, and inhibit the proliferation of these cells.

Key words: RNA, small interfering, Melanoma, Cell proliferation, CD147

陈翔, 林静, 林伟, 粟娟, 陈明亮, 施为, 谢红付, 陈服文. CD147 siRNA对恶性黑素瘤细胞株A375增殖及CD147表达的影响[J]. 中华皮肤科杂志, 2006,39(4):204-206. doi:

CHEN Xiang, LIN Jing, LIN Wei, SU Juan, CHEN Ming-liang, SHI Wei, XIE Hong-fu, CHEN Fu-wen. Effects of siRNA targeting CD147 gene on the expression of CD147 and the proliferation of malig- nant melanoma cell line A375[J]. Chinese Journal of Dermatology, 2006, 39(4): 204-206.doi:

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