中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (2): 86-88.

• 论著 • 上一篇    下一篇

磷酸化信号传导和转录激活因子3和上皮钙黏着蛋白在表皮肿瘤的表达

蔡绥勍1, 陈丽荣2, 王海军2, 姚丽芳2, 郑敏1   

  1. 1. 浙江大学医学院附属第二医院皮肤科 杭州 310009;
    2. 浙江大学医学院附属第二医院病理科 杭州 310009
  • 收稿日期:2004-02-05 出版日期:2005-02-15 发布日期:2005-02-15
  • 通讯作者: 郑敏,E-mail:minz@zju.edu.cn E-mail:minz@zju.edu.cn
  • 基金资助:
    浙江省卫生厅科研基金资助项目(2004A046)

Stat 3 Phosphorylation and E-cadherin Expression in Human Epidermal Non-melanoma Cutaneous Tumors

CAI Sui-qing1, CHEN Li-rong2, WANG Hai-jun2, YAO Li-fang2, ZHENG Min1   

  1. Dermatology Department, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, China
  • Received:2004-02-05 Online:2005-02-15 Published:2005-02-15

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摘要： 目的 探讨磷酸化信号传导和转录激活因子3(p-stat3)和E钙黏蛋白在表皮肿瘤的表达和意义。方法 免疫组化方法观察30例皮肤鳞状细胞癌(SCC)、20例基底细胞上皮瘤(BCC)、20例脂溢性角化病(SK)肿瘤细胞中p-stat3和E钙黏蛋白的表达,20例正常人皮肤为对照。结果 ①p-stat3在皮肤SCC、BCC中呈明显的上调表达,皮肤SCC中p-stat3的表达强度明显高于BCC(P<0.05);②p-stat3在皮肤SCC中的表达强度与肿瘤的分化程度有关(P<0.05),阳性表达率与肿瘤浸润的深度有关(P<0.05),与肿瘤的大小无关。③皮肤SCC中E钙黏蛋白的表达明显减弱(P<0.001),且皮肤SCC中E钙黏蛋白的表达强度比皮肤BCC也明显减弱(P<0.05)。在皮肤SCC中E钙黏蛋白的表达强度与肿瘤的分化程度有关,阳性表达率与肿瘤浸润的深度和肿瘤的大小无关。④皮肤SCC中,p-stat3和E钙黏蛋白的阳性表达强度具有负相关性,rs=-0.37,P<0.05。结论 p-stat3的表达异常可能在表皮肿瘤的发生中起重要作用。stat3的过度活化可能与皮肤SCC的侵袭性潜能密切相关。

关键词: 信号传导和转录激活因子3, 癌, 鳞状细胞, 癌, 基底细胞, 钙粘着糖蛋白类, 转录因子

Abstract: Objective To investigate stat 3 phosphorylation and E-cadherin expression and their clinical significance in human epidermal non-melanoma cutaneous tumors. Methods Immunohistochemistry technique was applied to measure the expression of p-stat 3 and E-cadherin protein in 30 cases of skin squamous cell carcinomas (SCC), 20 cases of basal cell carcinomas (BCC), 20 cases of seborrhoeic keratosis (SK) and 20 cases of normal subjects. Results ① p-stat 3 protein was significantly increased in SCC and BCC (P<0.001), and the expression of stat 3 in SCC was significantly higher than that in BCC (P<0.05). ② p-stat 3 expression in poorly differentiated SCC was higher than that in well differentiated SCC (P<0.05). The positive rate of p-stat 3 expression was correlated with the depth of tumor invasion (P<0.05), whereas there was no correlation between the positive rate and tumor size. ③ E-cadherin expression was significantly deceased in SCC (P<0.001), and E-cadherin expression was lower in SCC than that in BCC (P<0.05). In SCC, the intensity of E-cadherin expression was correlated with the extent of tumor differentiation, while there was no correlation between the expression and the depth of tumor invasion and the tumor size. ④There was a negative correlation between the expression intensity of p-stat 3 and E-cadherin in SCC (rs=-0.372, P<0.05). Conclusions The overexpression of p-stat 3 may play an important role in the development of epidermal tumors. The abnormal activation of stat 3 may be related to the potentials of tumor invasion in SCC.

Key words: Carcinoma, squamous cell, Carcinoma, basal cell, E-Cadherins, Transcription factors