中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (12): 721-723.

• 论著 • 上一篇    下一篇

半边旗提取物5F对系统性硬化病患者成纤维细胞凋亡的影响

肖佐环1, 张培华2, 吴志华2   

  1. 1. 中山医科大学附属第五医院皮肤科 珠海 519000;
    2. 广东医学院附属医院
  • 收稿日期:2003-12-22 出版日期:2004-12-15 发布日期:2004-12-15

Effects of Extract 5F from Pteris Semipinnata L. on the Apoptosis of Skin Fibroblasts in Patients with Systemic Scleroderma

XIAO Zuo-huan1, ZHANG Pei-hua2, WU Zhi-hua2   

  1. Department of Dermatology, the Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
  • Received:2003-12-22 Online:2004-12-15 Published:2004-12-15

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摘要： 目的 探讨半边旗提取物5F抑制系统性硬化病患者皮肤成纤维细胞增殖的机制.方法 用TUNEL法和荧光显微镜观察法测定半边旗提取物5F对成纤维细胞凋亡的影响.结果 半边旗提取物5F对患者皮肤成纤维细胞的凋亡具有显著的诱导作用,该作用在一定范围内随着药物浓度的增高和作用时间的延长而增强.结论 半边旗提取物5F可诱导系统性硬化病患者皮肤成纤维细胞的凋亡,从而抑制其增殖,减少其胶原蛋白的合成.

关键词: 硬皮病,系统性, 成纤维细胞, 细胞凋亡, 半边旗

Abstract: Objective To study the effects of extract 5F from Pteris semipinnata L.on the apoptosis of skin fibroblasts in patients with systemic scleroderma (SSc) and the possible mechanism of its inhibition on the proliferation of skin fibroblasts.Methods The cultured SSc skin fibroblasts were used as an experimental model, and the apoptosis of SSc skin fibroblasts before and afte r the extract 5F stimulation was detected by TUNEL assay and fluorescent microsc opy.Results The apoptosis of SSc skin fibroblasts was induced by the extract 5F, and the apoptosis was enhanced along with the increase of the concentration and incubation time of this compound within definite limits.Conclusions These results indicate that the extract 5F can induce the apoptosis of SSc skin fibro blasts, and significantly inhibit the proliferation and collagen synthesis in these cells.

Key words: Scleroderma,systemic, Fibroblast, Apoptosis, Pteris semipinnata