中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (12): 687-689.

• 论著 • 上一篇    下一篇

血小板衍化生长因子受体蛋白在瘢痕疙瘩中的表达

陈晓栋, 王强, 张国成, 叶顺章   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2002-12-16 出版日期:2003-12-15 发布日期:2003-12-15

Enhanced Expression of Platelet-derived Growth Factor Receptor Proteins in Keloids

CHEN Xiao-dong, WANG Qiang, ZHANG Guo-cheng, YE Shun-zhang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
  • Received:2002-12-16 Online:2003-12-15 Published:2003-12-15

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摘要： 目的 探讨血小板衍化生长因子受体(PDGFR)-α和-β在瘢痕疙瘩组织和瘢痕疙瘩来源成纤维细胞中的表达及其在瘢痕疙瘩发病中的作用.方法 应用免疫组化法检测15份瘢痕疙瘩和10份正常人皮肤标本PDGFR-α和-β蛋白的分布.体外原代培养成纤维细胞和蛋白质印迹法检测PDGFR蛋白的表达.结果 在瘢痕疙瘩组织中PDGFR-β表达明显增高,而PDGFR-α在瘢痕疙瘩中的表达似与瘢痕疙瘩的临床生长状态有关.在边缘充血、浸润生长明显的皮损,PDGFR-α呈强烈表达;而在边缘稳定、无明显浸润态势之皮损,PDGFR-α呈低表达.体外培养的成纤维细胞中,PDGFR-α比PDGFR-β表达更为丰富.结论 两种PDGFR的表达增高导致瘢痕疙瘩成纤维细胞对PDGF敏感性提高,决定了PDGF在瘢痕疙瘩发病机制中的作用.

关键词: 瘢痕疙瘩, 受体,血小板源生长因子, 成纤维细胞

Abstract: Objective To investigate the expression and localization of platelet-derived growth factor receptor-αand-β(PDGFR-αand-β)in keloid tissues and keloid-derived fibroblasts and to explore the role of PDGFR in the pathogenesis of keloid.Methods The distribution of PDGFR was determined by immunohistochemistry technique in15keloid lesions and 10 normal skin tissues.The expression of PDGFR protein in vitro was identified by Western blotting analysis.Results Strong staining of PDGFR-βwas located in keloid tissue.However,the expression of PDGFR-αin keloid tissue seemed to be related to the status of clinical proliferation.Strong expression of PDGFR-α was observed in the lesions with a congestive and invasive margin,whereas mild expression of PDDFR-α was found in lesions with a stable margin.Expression of PDGFR-αprotein was more abundant than that of PDGFR-β protein in fibroblasts in vitro.Conclusion The elevated levels of both types of PDGFR might facilitate their responses to PDGF in keloid fibroblasts,which seems to play an important role in the formation of keloid.

Key words: Keloid, Receptors,platelet-derived growth factor, Fibroblasts