中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (12): 690-692.

• 论著 • 上一篇    下一篇

高砷煤引起的砷中毒患者外周血红细胞血型糖蛋白A突变频率研究

徐海环1, 张信江1, 方宁2, 杜辉3, 周运书3, 袁伟1, 蒋玲1, 肖和1, 瓦庆彪1, 赵明亮1   

  1. 1. 遵义医学院附属医院皮肤性病科 563003;
    2. 遵义医学院附属医院细胞工程实验室;
    3. 贵州省黔西南州疾病控制中心
  • 收稿日期:2002-12-16 出版日期:2003-12-15 发布日期:2003-12-15
  • 通讯作者: 张信江,E-mail:xinjiangzhang@sohu.com E-mail:xinjiangzhang@sohu.com
  • 基金资助:
    贵州省省长资金(C-197);贵州省科技厅基金(C-220)

Frequencies of Mutations at Glycophorin-A Locus of Erythrocytes in Patients with High Arsenic Coal Poisoning

XU Hai-huan1, ZHANG Xin-jiang1, FANG Ning2, DU Hui3, ZHOU Yun-shu3, YUAN Wei1, JIANG Ling1, XIAO He1, WA Qing-biao1, ZHAO Ming-liang1   

  1. Department of Dermatology, Affiliated Hospital, Zunyi Medical College, Zunyi 563003, China
  • Received:2002-12-16 Online:2003-12-15 Published:2003-12-15

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摘要： 目的 检测高砷煤引起的砷中毒患者外周血红细胞血型糖蛋白A(GPA)的突变频率.方法 分离、固定40例高砷煤引起的砷中毒患者外周血红细胞,与荧光素标记的单抗结合后,采用流式细胞仪进行GPA突变频率分析.结果 40例高砷煤引起的砷中毒患者外周血红细胞GPA突变频率:NN突变频率为(21.23±13.97)×10-6,NO突变频率为(33.13±25.72)×10-6,MM突变频率为(110.90±63.58)×10-6,MO突变频率为(20.35±21.26)×10-6,GPA的突变频率明显高于正常人群(P<0.01).肿瘤组:NN突变频率为(31.50±16.13)×10-6,NO突变频率为(54.50±38.13)×10-6,MM突变频率为(159.33±66.22)×10-6,MO突变频率为(45.16±12.69)×10-6,GPA的突变频率明显高于非肿瘤组(P<0.01).结论 砷中毒可导致人类外周血红细胞GPA的突变.提示GPA的突变频率值可作为一项检测有否砷中毒及病变程度的指标.

关键词: 砷中毒, 红细胞, 血型糖蛋白, 突变

Abstract: Objectives To observe the frequencies of mutations at glycophorin A(GPA)of erythrocytes in patients with high arsenic coal poisoning(HACP)in comparison with normal controls.Methods The peripheral erythrocytes were isolated and immunolabelled,and were detected by flow cytometry in40patients and18normal adults.Results The mutation frequencies(MF) were(21.23±13.97)×10-6 for type NN,(33.13±25.72)×10-6 for type NO,(110.90±63.58)×10-6 for type MM,and(20.35±21.26)×10-6 for type MO of erythrocytes in patients with HACP,which were significantly higher than those in normal controls.The mutation frequencies were(31.50±16.13)×10-6 for type NN,(54.50±38.13)×10-6 for type NO,(159.33±66.22)×10-6 for type MM,and(45.16±12.69)×10-6 for type MO of erythrocytes in tumor group of HACP patients,which were significantly higher than those of non-tumor group of the patients.Conclusions Arsenic poisoning may induce the mutation at the glycophorin-A locus of erythrocytes,suggesting that arsenic may be one of potential mutagens.GPA-MF may serve as a parameter for the detection of patients with HACP.

Key words: Arsenic poisoning, Erythrocytes, Glycophorin, Mutation