中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 266-268.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者血清对正常造血干(祖)细胞的抑制作用

童秀珍1, 叶任高2, 罗绍凯1, 李娟1, 洪文德1   

  1. 1. 中山医科大学附属第一医院血液科 广州 510080;
    2. 中山医科大学附属第一医院肾内科 广州 510080
  • 收稿日期:2001-09-04 出版日期:2002-08-15 发布日期:2002-08-15
  • 通讯作者: 叶任高,中山医科大学附属第一医院肾内科,广州,510080
  • 基金资助:
    中山医科大学211重点科研基金资助(98150)

Suppression of Hematopoietic Stem/Pro genitor Cells by Sera from Patients with Systemic Lupus Erythematosus

TONG Xiuzhen1, YE Rengao2, LUO Shaokai1, LI Juan1, HONG Wende1   

  1. Department of Hematology, First Affiliated Hospital of Sun Yat-sen Medical University, Guangzho 510080, China
  • Received:2001-09-04 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 探讨系统性红斑狼疮(SLE)患者血象下降的发生机制.方法 用甲基纤维素集落培养方法观察有全血细胞减少的SLE患者的血清、去除IgG后的血清对正常骨髓造血干(祖)细胞粒细胞-巨噬细胞集落(CFU-GM)、红系祖细胞集落(CFU-E)的影响及SLE患者造血干(祖)细胞的增殖能力.结果 ①加入SLE患者的血清可见66.7%的正常骨髓CFU-GM、70%的CFU-E集落形成减少,与对照组比较差异均有显着性(P<0.001).②去除IgG后的血清组CFU-GM、CFU-E的产率高于未去除IgG血清组(P<0.001).③SLE患者骨髓CFU-GM、CFU-E集落数均明显低于正常对照组(P<0.01).结论 SLE患者的全血细胞减少与其血清自身抗体抑制造血干(祖)细胞有关,也与其本身干(祖)细胞的增殖分化能力下降有关.

关键词: 红斑狼疮,系统性, 血浆, IGG, 造血干细胞, 集落形成单位测定

Abstract: Objective To explore the mechanism of hematolo gic abnormality in patients with systemic lupus erythematosus(SLE).Methods Suppression of granulocytic and ery throid colony formation of bone marrow cells fromhealthy individuals was examined in vitro by using methylcell ulose culture with sera or IgGdelete d sera from patients with SLE.Results ①Both colony-forming units of erythr ocytes(CFU-E)(in 66.7% of samples tested)and granulocytes(CFU-GM)(in 70% of samples tested)of normal bone marrowcells were sign ificantly inhibited by sera frompatients with SLE(P<0.001).②In the IgG deleted group,the CFU-E an d CFU-GMyield rates were higher than those of the sera with IgG group.③Both the numbers of CFU-GM and CFU-E were markedly lower in patients with SLE than those in healthy controls.Conclusion The cytopenia may be related to the in hibition of progenitor cell growth by IgG autoan tibodies and decreased differentiation ability of hematopoietic stem/progenitor cells in patients with SLE.

Key words: Lupus erythematosus,systemic, Plasma, IgG, Hematopoieticstem cells, Colony-forming units assay