中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 263-265.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者Th1/Th2细胞及白介素12、18及其受体mRNA的表达

黎莉, 王晓栋, 陈顺乐, 沈南, 鲍春德, 顾越英   

  1. 上海第二医科大学附属仁济医院上海市风湿病研究所 200001
  • 收稿日期:2001-09-26 出版日期:2002-08-15 发布日期:2002-08-15
  • 基金资助:
    国家自然科学基金(39970696)

Expression of Th1/Th2 and Their Regu latory Cytokines in Systemic Lupus Erythematosus

LI Li, WANG Xiaodong, CHEN Shunle, SHEN Nan, BAO Chunde, GU Yueying   

  1. Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China
  • Received:2001-09-26 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 探讨未经药物治疗的初发系统性红斑狼疮患者Th1/Th2细胞亚群分布,白介素12、白介素18及其受体的基因表达.方法 运用三色荧光标记流式细胞术检测35例初发SLE患者、10例正常人Th1/Th2细胞亚群分布;ABI7700Real-TimePCR法同时检测38例患者和28例正常人IL-12、IL-18及其受体mRNA表达水平.结果 ①初发狼疮患者Th1较正常人明显减低(P<0.05),但Th1/Th2无显着性改变.②与正常人组相比,SLE组患者IL-12、IL-18mRNA及其受体表达较正常人明显降低(P值均<0.05);③面部红斑组患者Th1/Th2、IL-12P35较正常人组降低(P均<0.05);④RNP阳性组患者IL-12P40较正常人组升高,IL-12P35、IL-18较正常人组降低(P均<0.05).结论 SLE是一种以Th1细胞下降,Th2细胞相对占优势的自身免疫性疾病,源于诱导向Th1细胞分化的一系列细胞因子及其受体减少和细胞因子间失衡所致.

关键词: 红斑狼疮,系统性, Th1细胞, Th2细胞, 白细胞介素12, 白细胞介素18, 受体,白细胞介素

Abstract: Objective To analyze Th1/Th2subsets and their regulatory cytokines and cytokine r eceptors in recent onset SLE patients to determi ne the cause of immunological imbala nce in SLE.Methods Th1/Th2 cells were detected quantitatively by flow cytometry in patients with recent-onset SLE(n=35)and the controls(n=10).At the same time,IL-12,IL-12Rβ2 and IL-18,IL-18R mRNA expression we re tested by ABI7700 real time PCR in SLE patients(n=38/42)and the controls(n=28).Results No difference was found in Th1/Th2 ratio between SLE patients and the controls(P>0.05).However,Th1was decreased significantly in S LE patients than that in the normal controls(P<0.05).The cytokines including IL-12P35,IL-12P40、IL-18and their respective receptors IL-12Rβ2,IL-18R and IL-12P35/IL-10、IL-18/IL-10 were all significantly decreased in SLE group than those in the controls(P<0.05all).SLE patients with facial erythema presented significantly lower Th1/Th2 ratio and lower IL-12P35 mRNA exp ression than the controls did.Signi ficantly high IL-12P40 expression a s well as low IL-12P35 expression were found in SLE patients with positive RNP.Conclusions SLE is a disease with Th2 predominance due to low Th1 subset and decreased expressions of IL-12 and IL-12Rβ2,IL-18 and IL-18R mRNA.SLE patients with facial erythema and positive RNP may be the special subtype of SLE,for they have different Th distribution and the particular imbalance in cytokines expression.

Key words: Lupus erythematosus,systemic, Th1cells, Th2cells, Interleukine 12, I nterlukine 18, receptors,interleukine