中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (6): 404-406.

• 论著 • 上一篇    下一篇

北京地区育龄妇女泌尿生殖道沙眼衣原体感染状况及基因分型初步研究

徐克沂1, 李敏1, 伦文辉1, Carolyn M Black2   

  1. 1. 北京地坛医院性传播疾病中心 100011;
    2. 美国疾病控制中心性传播疾病部
  • 收稿日期:1999-10-28 出版日期:2000-12-15 发布日期:2000-12-15

Study of Chlamydial Urogenital Infection and MOMP Gene Variants in Child-bearing Women in Beijing

XU Keyi1, LI Min1, LUN Wenhui1   

  1. Beijing Ditan Hospital STD Center, 100011
  • Received:1999-10-28 Online:2000-12-15 Published:2000-12-15

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摘要： 目的 对北京地区育龄妇女沙眼衣原体的感染状况及基因分型进行初步研究。方法 利用胶体金免疫法对177例计划生育门诊及妇科体检的育龄妇女进行筛查,对9例沙眼衣原体感染阳性者的拭子标本直接进行套式PCR扩增主要外膜蛋白(MOMP)基因。将扩增的DNA进行限制性内切酶长度多态性分析,并对MOMP基因易变区VS1、VS2、VS3、VS4进行基因序列分析。结果 177名育龄妇女有9例沙眼衣原体感染阳性,9份标本均扩增出1.1kb目的DNA,经限制性内切酶长度多态性分析,4例确定为F型,3例为E型,2例为D型。利用373A自动序列分析仪,对MOMP基因易变区VS1、VS2、VS3、VS4进行基因序列分析,与标准序列比较发现,2例为D型,3例为E型,4例为F型,与限制性内切酶长度多态性分析结果相同,但其中4例有MOMP基因变异存在。结论 北京地区健康育龄妇女存在一定比例的沙眼衣原体感染,利用对MOMP基因的多态性分析可以对沙眼衣原体的流行情况作出分析。

关键词: 衣原体, 沙眼, 主要外膜蛋白, 多态性, 限制性片段长度, 序列分析, DNA

Abstract: Objective To investigate Chlamydia trachomatis infection and gene variants in child-bearing women in Beijing. Methods Endocervical samples were collected from 177 patients in a family planning clinic and a gynecologic clinic. C.trachomatis infection was screened with a fast diagnostic kit. Major outer membrane protein(MOMP) gene was amplified by a nested PCR. A 1.1kb amplified product was obtained and studied by restriction fragment length polymorphism analysis(RFLP). Variant domains of MOMP gene VS1,VS2,VS3,VS4 were sequenced with 373A automated sequencing system. Results C. trachomatis was found in 9 of 177 samples. Four samples were identified as F genotype, 3 E genotype and 2 D genotype. Variant domains of MOMP gene, VS1, VS2, VS3, VS4 were sequenced and the results were consistent with those of RFLP, however, mutations were found in MOMP gene in 4 samples. Conclusion C.trachomatis urogenital infection has been identified in a portion of child-bearing women in Beijing. C.trachomatis can be well genotyped with RFLP or gene sequencing of MOMP gene.

Key words: Chlamydia trachomatis, Major outer membrane protein, Polymorphism, restriction fragment length, Sequence analysis, DNA