中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (3): 162-164.

• 论著 • 上一篇    下一篇

生殖道沙眼衣原体细胞培养和血清学研究

倪安平1, 沈浣2, 何海英1, 胡美兰2, 郭建坤4, 廖绍荣3, 盖明英1, 郑和义1, 王家璧1   

  1. 1. 中国医学科学院, 中国协和医科大学北京协和医院 北京 100730;
    2. 北京医科大学人民医院;
    3. 北京市朝阳区小庄医院;
    4. 黑龙江省第三建筑公司中心医院
  • 收稿日期:1999-10-18 出版日期:2000-06-15 发布日期:2000-06-15

Detection of Genital C.trachomatis Infection by Cell Culture and Serology

NI Anping1, SHEN Huan2, HE Haiying1   

  1. Peking Union Medical College Hospital, CAMS and PUMC, Beijing 100730
  • Received:1999-10-18 Online:2000-06-15 Published:2000-06-15

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摘要： 目的 研究孕妇、盆腔炎患者和STD门诊男女就诊者生殖道沙眼衣原体病原学、血清学及两者之间的关系,并与对照人群进行比较.方法 细胞培养检测生殖道沙眼衣原体;酶免疫和微量免疫荧光试验方法检测血清沙眼衣原体抗体.结果 孕妇、盆腔炎患者和STD男女就诊者生殖道沙眼衣原体阳性率分别为3.3%,6.7%,20.4%和15.6%;血清沙眼衣原体酶免疫法检测IgG/微量免疫荧光试验方法IgG抗体阳性率分别46.7%/11.9%,70.2%/39.9%,73.5%/52.4%和75.4%/63.1%,统计学上均极为显著高于对照人群(孕妇组除外).女性生殖道沙眼衣原体阳性者比阴性者统计学上具有更高的血清沙眼衣原体抗体,男性则无这种差异.8例存在沙眼衣原体急性抗体.结论 应常规筛查PID患者沙眼衣原体,但尚无需筛选孕妇;女性宫颈沙眼衣原体感染与血清学具有重要的关联;血清学无助于生殖道沙眼衣原体感染的诊断.

关键词: 衣原体,沙眼, 细胞,培养的, 血清学

Abstract: Objectives To determine the prevalences of genital C.trachomatis and serum chlamydial antibodies in pregnant women,PID patients and the patients attending STD clinic,and to compare the chlamydial serology with control subjects.Methods Genital C.trachomatis was detected by cell culture,and serum chlamydial antibodies were measured by EIA and MIF.Results Genital C.trachomatis was found in 3.3% of pregnant women,6.7% of PID patients,20.4% or 15.6% of male or female patients attending STD clinic,respectively.Prevalence of chlamydial EIA-IgG/MIF-IgG antibodies was 46.7%/11.9% in pregnant women,70.2%/39.9% in PID patients,73.5%/52.4% and 75.4%/63.1% in male and female STD patients,which were statistically higher than those of controls (except pregnant women).Women,rather than men,with genital C.trachomatis were more likely to have serum chlamydial antibodies than those without infection.Eight subjects were measured with acute antibodies to C.trachomatis.Conclusions There is strong relation between cervical C.trachomatis and serum chlamydial antibodies.Serum antibodies to C.trachomatis are of no help for the diagnosis of genital C.trachoamtis infections.Routine screening of C.trachomatis is needed for patients with PID but not for pregnant women.

Key words: Chlamydia trachomatis, Cells,cultured, Serology