中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (6): 391-392.

• 论著 • 上一篇    下一篇

长波紫外线照射对培养的正常人黑素细胞增殖及黑素合成的影响

项蕾红, 郑志忠, 祝绿川, 廖康煌   

  1. 上海医科大学附属华山医院皮肤科 200040
  • 收稿日期:1999-07-06 出版日期:1999-12-15 发布日期:1999-12-15

Effects of UVA Radiation on Proliferation and Melanogenesis of Normal Human Melanocytes in Vitro

XIANC Leihong, ZHENC Zhizhong, ZHU Luchuan   

  1. Department of Dermatology, Hua Shan Hospital, Shanghai Medical University, Shanghai 200040
  • Received:1999-07-06 Online:1999-12-15 Published:1999-12-15

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摘要： 目的 了解UVA对黑素细胞增殖及黑素合成的影响.方法 采用体外细胞培养技术、MTT法及NaOH法研究不同剂量UVA照射对黑素细胞增殖和黑素合成的作用.结果 随着照射时间的增加,黑素细胞的增殖率增加,黑素合成量也增加.结论 照射剂量在3.6J/cm2以内时,黑素细胞增殖率逐步上升,以后趋向稳定.在测定的照射剂量内,黑素合成量与照射剂量呈正相关.

关键词: 紫外线, 黑素细胞, 细胞,培养的

Abstract: Objective To investigate the effects of UVA on proliferation and melanogenesis of normal human melanocytes.Methods Normal human melanocytes were cultured from foreskins of healthy men.MTT spectrophotometry and NaOH assay were applied to determine the proiferation of melanocytes and the synthesis of melanin.Results & Conclusion It was found that UVA could induce the proliferation and melanogenesis of melanocytes in a dose-dependent manner.However, the effect of UVA radiation on the induction of melanocyte proliferation was limited.

Key words: Ultraviolet rays, Melanocyte, Cells,cultured