中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (4): 253-255.

• 论著 • 上一篇    下一篇

皮肤鳞状细胞癌分级与p53蛋白和增殖细胞核抗原表达及与巨噬细胞反应的关系

王建力, 陈莉   

  1. 南通医学院附属医院皮肤科, 江苏 226001
  • 收稿日期:1996-07-11 修回日期:1996-11-15 出版日期:1997-08-15 发布日期:1997-08-15

Expression of p53, PCNA and Stroma Macrophage in Relation to the Degree of Differentiation in Squamous Cell Carcinoma of the Skin

Wang Jianli, Chen Li   

  1. Department of Dermatology, Affiliated Hospital, Nantong Medical College, Nantong 226001
  • Received:1996-07-11 Revised:1996-11-15 Online:1997-08-15 Published:1997-08-15

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摘要： 免疫组织化学法检测50例皮肤鳞状细胞癌组织标本中p53蛋白,增殖细胞核抗原(PCNA)的表达及其癌间质中巨噬细胞反应,在原位客观地观察癌细胞增殖活性,间质巨噬细胞反应与癌分化、分级的关系。结果表明本组病例p53蛋白检出率为56%,各级癌中p53蛋白阳性检出率无显著性差异,但p53蛋白阳性表达细胞数与癌分级呈正相关。癌中PCNA阳性率为100%,阳性细胞数与癌分级也呈正相关。癌中p53蛋白表达与PCNA表达呈平行关系。癌间质巨噬细胞规律性排列于癌活跃增殖的巢边缘区,其浸润量与癌分级呈负相关。结论:p53蛋白、PCNA表达随癌分级从Ⅰ级到Ⅳ级逐步增强,反映了癌细胞增殖、分化的规律,间质巨噬细胞在一定程度上抑制癌细胞增殖。

关键词: 癌, 鳞状细胞, p53蛋白, PCNA, 巨噬细胞

Abstract: The expressions of p53 protein, PCNA and macrophage count in 50 cases of skin squamous cell carcinoma were studied by LSAB immunostaining of monoclonal antibodies (PC-10, CD68 and Do-7). The relationship between the proliferation of cancer cell and the count of stroma macrophage to the degree of differentiation was studied and observed in situ. The results showed that the positive rates of expression of both p53 protein and PCNA in the squamous cell carcinoma were 56% and 100%, respectively. The distribution and strength of the expression of p53 protein and PCNA were positively correlated to the degree of cell differentiation. Macrophoge regularly located in the margin of cancer nest. The macrophage count was reversely correlated to the degree of differentiation. It is concluded that the degree of differentiation is parallel to the expression of p53 protein and PCNA, whereas, the macrophages in the stroma to some extent inhibits the cancer cell proliferation.

Key words: Carcinoma, squamous cell, Protein p53, PCNA, Macrophages