中华皮肤科杂志 ›› 2022, Vol. 55 ›› Issue (5): 425-429.doi: 10.35541/cjd.20201009

• 研究报道 • 上一篇    下一篇

Q开关Nd:YAG 1 064 nm激光对糠秕马拉色菌细胞活性、蛋白酶活性及结构的影响

谈桂其    钟静    张丽丹    李晓辉    彭洁雯    翁智胜   

  1. 广州医科大学附属第三医院皮肤科,广州  510150
  • 收稿日期:2020-10-15 修回日期:2021-05-26 发布日期:2022-04-29
  • 通讯作者: 翁智胜 E-mail:wzs338@21cn.com

Effect of 1 064-nm Q-switched Nd:YAG laser on cell viability, protease activity and structures of Malassezia furfur

Tan Guiqi, Zhong Jing, Zhang Lidan, Li Xiaohui, Peng Jiewen, Weng Zhisheng    

  1. Department of Dermatology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China
  • Received:2020-10-15 Revised:2021-05-26 Published:2022-04-29
  • Contact: Weng Zhisheng E-mail:wzs338@21cn.com

摘要: 【摘要】 目的 探讨不同能量Q开关Nd:YAG 1 064 nm激光对糠秕马拉色菌的细胞活性、蛋白酶活性及菌体结构的影响。方法 选用糠秕马拉色菌标准株,分别予以Q开关Nd:YAG 1 064 nm激光0(对照组)、500、600、700、800、900 mJ能量照射,培养7 d后测量各组菌落直径及菌落数,评估菌体细胞活性,采用全脂牛奶平板法测定蛋白酶活性,透射电镜观察各组菌体超微结构。多组间比较采用单因素方差分析,组间两两比较采用LSD-t检验,激光能量与菌落直径、菌落数及蛋白酶活性的相关性采用Pearson相关分析。结果 对照组及500、600、700、800、900 mJ组菌落直径(4.05 ± 0.69、3.76 ± 0.51、3.28 ± 0.41、3.09 ± 0.72、2.54 ± 0.64、2.43 ± 0.41 mm)、菌落数(4 787 ± 597、4 287 ± 761、1 879 ± 275、1 082 ± 248、209 ± 42、72 ± 31)差异均有统计学意义(F值分别为14.83、231.85,P < 0.05),600、700、800、900 mJ组菌落直径、菌落数均小于对照组(均P < 0.05)。激光能量与菌落直径和菌落数均呈负相关(r值分别为 -0.67、-0.91,P < 0.05)。对照组、500 mJ组、700 mJ组、900 mJ组蛋白酶活性差异有统计学意义(F = 346.60,P < 0.05),700 mJ、900 mJ组蛋白酶活性均低于对照组(P < 0.05)。激光能量与蛋白酶活性呈负相关(r = -0.94,P < 0.05)。透射电镜下观察到对照组菌体结构完整,500 mJ组菌体结构较完整,600 ~ 900 mJ组结构明显受破坏,且激光能量越大菌体结构破坏越严重。结论 Q开关Nd:YAG 1 064 nm激光可影响糠秕马拉色菌细胞及蛋白酶活性,破坏菌体结构。

关键词: 马拉色霉菌属, 激光, 固体, Nd-YAG激光, 细胞结构, 细胞活性, 蛋白酶活性

Abstract: 【Abstract】 Objective To investigate the effect of 1 064-nm Q-switched Nd:YAG laser at different energy settings on cell viability, protease activity and structures of Malassezia furfur. Methods Cultured standard strains of Malassezia furfur were divided into several groups to be irradiated with 1 064-nm Q-switched Nd:YAG laser at different energies of 0 (control group), 500, 600, 700, 800 and 900 mJ, respectively. Then, fungal suspensions in the above groups were inoculated onto the Leeming & Notman medium separately. After 7-day culture, the diameter and number of colonies were measured to evaluate the fungal cell viability, the protease activity was measured by using the whole-milk plate medium, and the ultrastructure of Malassezia furfur in each group was observed by transmission electron microscopy. One-way analysis of variance was used for comparisons among multiple groups, least significant difference-t test for multiple comparisons, and Pearson correlation analysis for analyzing correlations of laser energy with colony diameter, colony number and protease activity. Results The colony diameter and number both significantly differed among the control group, 500-, 600-, 700-, 800- and 900-mJ groups (colony diameter: 4.05 ± 0.69, 3.76 ± 0.51, 3.28 ± 0.41, 3.09 ± 0.72, 2.54 ± 0.64 and 2.43 ± 0.41 mm, respectively; colony number: 4 787 ± 597, 4 287 ± 761, 1 879 ± 275, 1 082 ± 248 and 209 ± 42, 72 ± 31 colony-forming units, respectively; F = 14.83, 231.85, respectively, both P < 0.05), and were significantly lower in the 600-, 700-, 800- and 900-mJ groups than in the control group (all P < 0.05). The laser energy was negatively correlated with the colony diameter and number (r = -0.67, -0.91, respectively, both P < 0.05). The protease activity significantly differed among the control group, 500-, 700- and 900-mJ groups (F = 346.60, P < 0.05), and was significantly lower in the 700- and 900-mJ groups than in the control group (both P < 0.05). There was a negative correlation between the laser energy and protease activity (r = -0.94, P < 0.05). Transmission electron microscopy showed intact fungal structures in the control group, relatively intact fungal structures in the 500-mJ group, and obviously damaged fungal structures in the 600- - 900-mJ groups, and the greater the laser energy, the more severely the fungal structures were damaged. Conclusion The 1 064-nm Q-switched Nd:YAG laser could affect the cell viability of and protease activity in Malassezia furfur, and damage the fungal structures.

Key words: Malassezia, Lasers, solid-state, Nd-YAG lasers, Cellular structures, Cell activity, Protease activity

引用本文

谈桂其 钟静 张丽丹 李晓辉 彭洁雯 翁智胜. Q开关Nd:YAG 1 064 nm激光对糠秕马拉色菌细胞活性、蛋白酶活性及结构的影响[J]. 中华皮肤科杂志, 2022,55(5):425-429. doi:10.35541/cjd.20201009

Tan Guiqi, Zhong Jing, Zhang Lidan, Li Xiaohui, Peng Jiewen, Weng Zhisheng . Effect of 1 064-nm Q-switched Nd:YAG laser on cell viability, protease activity and structures of Malassezia furfur[J]. Chinese Journal of Dermatology, 2022, 55(5): 425-429.doi:10.35541/cjd.20201009