Abstract: JING Wei-fang, LI Chun-li, GUO Ya-li, LIU Yuan-jun, QI Yu-qing, LIU Quan-zhong, WANG Hui-ping*. *Department of Dermatology and Venereology, General Hospital of Tianjin Medical University, Tianjin 300052, China Corresponding author: WANG Hui-ping, Email: huiping1208@163.com 【Abstract】 Objective To investigate the relationship of point mutations in ERG11, TAC1, MRR1 and UPC2 genes to fluconazole resistance in Candida albicans. Methods This study included 11 fluconazole-resistant Candida albicans strains. DNA was extracted from these Candida isolates by urea pyrolysis method. PCR was carried out to amplify the full-length EGR11 gene, as well as the corresponding fragments of TAC1, MRR1 and UPC2 genes followed by DNA sequencing. Results Eight mutations were detected in the ERG11 gene, including D116E, Y132H, Y132F, K143Q, T229A, E266D, G448E and G464S. Among them, K143Q was a novel mutation and had not been reported. A G648D mutation was detected in the UPC2 gene. No mutations were detected in the TAC1 or MRR1 gene. Conclusions There is a definite association between fluconazole resistance and ERG11 mutations, while further research is warranted to clarify the relationship between the mutations of TAC1, MRR1, UPC2 genes and resistance to fluconazole.