Abstract: Immunoblotting(IB) technique has been frequently used to detect bullous pemphigoid (BP) antibodies in BP patient.It is important to extract the antigen proteins fully and intactly in the base membrane zone(BMZ) of normal human epidermis.The simple method of extracting antigens will make IB techniqne easy.Three different methods of extracting antigen proteins were compared.Using IB for detecting BP antibodies,it has been found that the results of epidermal extract preparations are basically similar.However,one of these methods (No.1) is more convenient and may obtain more amounts of antigens.Another method(No.3) is identical to that of extracting antigens in dermis for detecting epidermolysis bullosa acquista (EBA) antibodies.Therefore,in using IB technique to distinguish BP from EBA,one can apply method No.3.

Key words: Antigens, Pemphigoid, Immunoblotting