Abstract: The effect of povidone iodine solution(PIS)on the metabolic activity of Candida albicans and the effect of PIS treated C.albicans to cultured human keratinocytes were studied by using the Alamar blue fluorescence assay.It was found that PIS inhibited the metabolic activity of Candida albicans with a dose-dependent manner,and that the LD₅₀ of PIS for Candida albicans is 0.075%.After treating for 30 min by 2% PIS/PBS(-),Candida albicans almost lost the adherence and invasion to the cultured human keratinocytes,the wall of yeast cells became uneven and shrunken,the intracellular structures became obviously indistinct,and the yeasts could not develop to hypha form.Candida albicans,treated with 2% PIS in PBS(-)for 30 min or with 10% PIS Sabouraud's liquid medium for 33 hr,did not form colony when it was inoculated to Sabouraud's dextrose agar.This result suggests that the fungicidal effective of PIS is influenced by its solvent.

Key words: Povidone iodine, Candida albieans, Keratinocytes