Abstract: Objective To investigate the expression of ICAM-1 in human malignant melanoma (MM)cell lines and its clinical significance.Methods Human MM cell lines MM8.1 were cultured and analyzed by reverse transcription polymerase chain reaction(RT-PCR), the cell shape,cell growth ability and ICAM-1 by mRNA expression under the stimulation of hyperthermia or cytokines were observed.Results Hyperthermia、γ-IFN and TNF-α could change cell shape,reduce cell growth ability and increase the expression of ICAM-1.IL-2 could not influence the cell shape and the expression of ICAM-1.Conclusion The expression of ICAM-1 in MM cell lines may be correlated with the cell destruction,which provides theoretical basis of supplementary therapy for MM with hyperthermia、IFNγ and TNFα.

Key words: Melanoma, Cytokine, Intercellular adhesion molecule-1, Reverse transcription polymerase chain reaction