Chinese Journal of Dermatology ›› 2012, Vol. 45 ›› Issue (2): 138-139.
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Juan PANG 2,yang guoling
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Abstract:
Objective To clone the full-length cDNA of Microsporum canis membrane protein PQ-loop repeat protein (PQ-LRP) gene, so as to investigate the roles of PQ-LRP in the pathogenesis of tinea capitis. Methods A Microsporum canis strain (A518) from a patient with tinea capitis served as the experimental strain. Rapid cDNA end amplification (RACE) was performed to clone the full length cDNA sequence of PQ-LRP gene. Bioinformatics methods were used to make a preliminary functional analysis of the gene. Results The cDNA of PQ-LRP gene was obtained with a full length of 1522 bp, including the 5′ untranslated region (49 bp), coding region (1080 bp) and 3′ untranslated region (393 bp). The coding region encoded a protein precursor including 359 amino acid residues. The cloned cDNA of PQ-LRP gene shared an 81% nucleotide identity with that of Trichophyton tonsurans and a 79% nucleotide identity with that of Trichophyton rubrum. Conclusions The full-length cDNA of Microsporum canis membrane protein PQ-LRP gene has been successfully cloned, which will provide an important basis for further researches into the roles of PQ-LRP in Microsporum canis-associated diseases.
Key words: SMART-RACE
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Juan PANG yang guoling. Cloning of full-length cDNA of Microsporum canis membrane protein PQ-loop repeat protein gene[J].Chinese Journal of Dermatology, 2012, 45(2): 138-139.
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