Abstract: Objective Use of Denaturing High-Performance Liquid chromatography for Rapid Detection and Identification of seven Common Pathogenic Candida Species. Methods PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 7 type strains of Pathogenic Candida Species，and the single and mixed products were analyzed respectively. Results Both single and mixed products show unique peak profiles，The whole process tool about 7 h to complete the identification. Conclusion PCR-DHPLC is fast, simple, specific and high-through for rapid detection and identification of common pathogenic Candida species. Moreover, it can detect different species of mixed samples, which may provide a reliable approach to clinical diagnosis of deep Candida infection.

Key words: Candida, Denaturing High-Performance Liquid chromatography, polymerase chain reaction