Abstract:

Wang Xiaohua, Rao Lang, Yu Huanhuan, Chen Wenjing, Zheng Daocheng, Chen Yongfeng Department of Dermatology, Guangdong Provincial Dermatology Hospital, Guangzhou 510091, China （Wang XH, Chen WJ, Zheng DC, Chen YF）; Department of Dermatology, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China （Rao L）; Department of Dermatology, Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450002, China （Yu HH） Corresponding author: Chen Yongfeng, Email: gdcyf@163.com 【Abstract】 Objective To investigate microRNA （miRNA） profiles in peripheral blood and skin lesions of patients with psoriasis vulgaris （PV）, and to seek miRNAs consistently expressed in peripheral blood and skin lesions. Methods A miRNA microarray was used to screen differentially expressed miRNAs in skin lesions and peripheral blood samples between 17 patients with PV and 4 healthy human controls, and real-time fluorescence-based quantitative PCR （RT-qPCR） to verify the differentially expressed miRNAs. The correlations of these differentially expressed miRNAs with psoriasis area and severity index （PASI） score were assessed by Pearson correlation analysis. Results The Agilent human miRNA microarray profiling revealed 15 miRNAs consistently expressed in skin lesions and peripheral blood of patients with PV. Of the 15 miRNAs, 7 were verified as consistently expressed miRNAs by RT-qPCR. Among the 7 miRNAs, the intensity of miR-30e-5p, miR-192-5p, miR-17-3p and miR-1227-5p was negatively correlated with PASI scores （all P < 0.05）, while that of miR-125b-5p, miR-642a-5p and miR-29a-5p was uncorrelated with PASI scores （all P > 0.05）. Conclusion Some miRNAs are consistently expressed in skin lesions and plasma of PV patients, which are expected to serve as biomarkers for evaluation of psoriasis severity.