Abstract:

Cao Yuping, Shen Yongnian, Lyu Guixia, Wang Le, Zeng Rong, Li Lingjun, Ma Pengcheng, Liu Weida Department of Sexually Transmitted Disease, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing 210042, China （Cao YP）; Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing 210042, China （Shen YN, Lyu GX, Liu WD）; Division of Medical Affairs, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing 210042, China （Wang L, Zeng R）; Pharmacal Research Laboratory, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing 210042, China （Li LJ, Ma PC） Corresponding authors: Liu Weida, Email: liumyco@hotmail.com; Ma Pengcheng, Email: mpc815@163.com 【Abstract】 Objective To establish a Trichophyton mentagrophytes-infected tissue-engineered skin model in vitro. Methods Primary fibroblasts were cultured with the presence of type Ⅰ bovine collagen for 3 days to form tissue-engineered dermis. Then, primary keratinocytes and melanocytes were co-cultured on the surface （namely air-liquid interface) of the artificial dermis for another 12 days to form a tissue-engineered skin model. Five microliters of fresh Trichophyton mentagrophytes suspensions were used to infect the tissue-engineered skin model. Hematoxylin and eosin （HE) staining and periodic acid-Schiff （PAS） staining were conducted to observe the structure of the cultures at 12, 24, 48 and 72 hours separately after the infection. Results A tissue-engineered dermal equivalent with a compact and uniform structure was formed by the culture of fibroblasts with the presence of typeⅠbovine collagen, and a well-stratified and -structured epidermis was formed by cocultured keratinocytes and melanocytes on the surface of the dermis. After infection with Trichophyton mentagrophytes, the structure of tissue-engineered skin was gradually destroyed over time, and epidermal destruction was severest at 48 hours; the entire structure of the tissue-engineered skin was replaced by hyphae and spores at 72 hours. Conclusion A Trichophyton mentagrophytes-infected tissue-engineered skin model has been preliminarily established.