Abstract: Zhang Hongchang, Zuo Lichun, Sun Haicai, Lyu Guangfu, Li Na, Lin Zhe Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China（Zhang HC, Lyu GF, Li N, Lin Z）; Changchun Sino-German Orthopaedic Hospital, Changchun 130051, China （Zuo LC, Sun HC） Corresponding author: Lin Zhe, Email: linzhe1228@163.com 【Abstract】 Objective To evaluate effects of Lycium barbarum polysaccharide （LBP） on of vascular endothelial growth factor （VEGF） and hypoxia inducible factor-1α（HIF-1α） in ultraviolet B （UVB）-radiated HaCaT cells. Methods Conventionally cultured HaCaT cells were divided into control group and LBP groups, which were firstly treated with DMEM, 12.5, 25.0, 50.0 and 100 μg/ml LBP solution respectively for 4 hours, and then were irradiated by UVB at different intensity of 0, 20, 40, 60 mJ/cm2 separately. After 24-hour continuing culture, CCK-8 assay was performed to determine the cell survival rate, and an enzymatic-biochemical method to estimate the activity of superoxide dismutase（SOD）. RT-PCR and Western blot analysis were conducted to measure the mRNA and protein of HIF-1α and VEGF respectively. Results Compared with the control group at the same UVB radiation dose, the 12.5-, 25.0- and 100.0-μg/ml LBP groups showed different extents of increase in survival rates of UVB-radiated cells （P < 0.05）, and the 50.0-μg/ml LBP group showed the highest cell survival rate （P < 0.01）. Among all the LBP groups, SOD activity was highest in the 50.0-μg/ml LBP group （P < 0.01）. Along with the increase of UVB radiation dose, the mRNA and protein of HIF-1α and VEGF all gradually increased. Compared with the control group, the 50.0-μg/ml LBP group could effectively reduce the mRNA and protein of HIF-1α and VEGF in HaCaT cells （all P < 0.05）. Conclusion LBP may play a role in protecting cells from UVB radiation-mediated damage, likely by influencing the mRNA and protein of HIF-1α and VEGF in HaCaT cells.