Abstract: Jiang Weiwei, Zhu Yinghua, Yang Min, Yue Hongyuan, Wang Hongmei, Yang Haiqiong, Jiao Yang Dalian Medical University, Dalian 116021, China （Jiang WW, Jiao Y）; Department of Dermatology, Dalian Dermatosis Hospital, Dalian 116021, China （Zhu YH, Yang M, Yue HY, Wang HM, Yang HQ） Corresponding author: Zhu Yinghua, Email: zyh-office@163.com 【Abstract】 Objective To detect levels of C-X-C chemokine receptor type 5 （CXCR5） and inducible costimulator （ICOS） in blister fluid of patients with bullous pemphigoid （BP）, and to explore their significance in the pathogenesis of BP. Methods Blister fluid samples were collected from 15 patients with BP （experimental group） and 15 patients with second-degree burns （control group）. Enzyme-linked immunosorbent assay （ELISA） was performed to detect the levels of CXCR5 and ICOS in the 2 groups. Results The level of CXCR5 was significantly higher in the experimental group than in the control group （219 ± 145.31 vs. 147 ± 23.83 ng/L, t = 4.577, P < 0.05）, while no significant difference in the ICOS level was observed between the 2 groups （30.18 ± 14.86 vs. 21.43 ± 5.32 ng/L, t = 1.628, P > 0.05）. Conclusion The of CXCR5 may be associated with the occurrence of BP, but further researches are needed to determine the relationship between ICOS and the occurrence of BP.